Matches in SemOpenAlex for { <https://semopenalex.org/work/W2038022611> ?p ?o ?g. }
Showing items 1 to 58 of
58
with 100 items per page.
- W2038022611 abstract "The neurophysins (NP) are proteins which function in neurohypophyseal peptide hormone transport and storage. We have been investigating the fluorescence properties of the single tyrosine residue (Tyr<SUP>49</SUP>) of bovine NPs. Since the intensity of Tyr<SUP>49</SUP> fluorescence increases on ligand binding, measurements have been made with and without small peptides which bind at the hormone binding site. Steady-state iodide quenching produces Stern-Volmer plots with downward and upward curvature for NP and the NP:Phe-PheNH<SUB>2</SUB> complex, respectively. Non-linear Stern-Volmer plots can result from multiple conformations of the solvated protein, multiple environments for the rotational isomers of the phenol side chain (rotamers), as well as from dynamic and static interactions of Tyr<SUP>49</SUP> with the quencher. To help explain the steady-state data, time-resolved fluorescence quenching studies have been performed. Both NP and the NP:Phe-PheNH<SUB>2</SUB> complex exhibit fluorescence intensity decays that can be fit by the sum of three exponentials that consist of the same time constants but with different weighting (amplitude) terms. With increasing [I<SUP>-</SUP>], the decay parameters for Tyr<SUP>49</SUP> in NP are consistent with a multiple species model due to rotamers of Tyr<SUP>49</SUP>, the amplitudes are quencher independent and the lifetimes result in linear Stern-Volmer plots. The quenching studies on the NP:PhePheNH<SUB>2</SUB> binary complex, however, cannot be analyzed in terms of a specific model because the fractional intensities of two of the exponential components are small." @default.
- W2038022611 created "2016-06-24" @default.
- W2038022611 creator A5002779864 @default.
- W2038022611 creator A5034574047 @default.
- W2038022611 creator A5075023574 @default.
- W2038022611 date "1992-04-01" @default.
- W2038022611 modified "2023-09-23" @default.
- W2038022611 title "<title>Perturbations of the local environment of the single tyrosine residue in neurophysin upon ligand binding as determined by steady-state and time-resolved fluorescence quenching</title>" @default.
- W2038022611 doi "https://doi.org/10.1117/12.58251" @default.
- W2038022611 hasPublicationYear "1992" @default.
- W2038022611 type Work @default.
- W2038022611 sameAs 2038022611 @default.
- W2038022611 citedByCount "0" @default.
- W2038022611 crossrefType "proceedings-article" @default.
- W2038022611 hasAuthorship W2038022611A5002779864 @default.
- W2038022611 hasAuthorship W2038022611A5034574047 @default.
- W2038022611 hasAuthorship W2038022611A5075023574 @default.
- W2038022611 hasConcept C113196181 @default.
- W2038022611 hasConcept C121332964 @default.
- W2038022611 hasConcept C121745418 @default.
- W2038022611 hasConcept C178790620 @default.
- W2038022611 hasConcept C185592680 @default.
- W2038022611 hasConcept C18705241 @default.
- W2038022611 hasConcept C2778639546 @default.
- W2038022611 hasConcept C32909587 @default.
- W2038022611 hasConcept C43617362 @default.
- W2038022611 hasConcept C62520636 @default.
- W2038022611 hasConcept C8010536 @default.
- W2038022611 hasConcept C91881484 @default.
- W2038022611 hasConceptScore W2038022611C113196181 @default.
- W2038022611 hasConceptScore W2038022611C121332964 @default.
- W2038022611 hasConceptScore W2038022611C121745418 @default.
- W2038022611 hasConceptScore W2038022611C178790620 @default.
- W2038022611 hasConceptScore W2038022611C185592680 @default.
- W2038022611 hasConceptScore W2038022611C18705241 @default.
- W2038022611 hasConceptScore W2038022611C2778639546 @default.
- W2038022611 hasConceptScore W2038022611C32909587 @default.
- W2038022611 hasConceptScore W2038022611C43617362 @default.
- W2038022611 hasConceptScore W2038022611C62520636 @default.
- W2038022611 hasConceptScore W2038022611C8010536 @default.
- W2038022611 hasConceptScore W2038022611C91881484 @default.
- W2038022611 hasLocation W20380226111 @default.
- W2038022611 hasOpenAccess W2038022611 @default.
- W2038022611 hasPrimaryLocation W20380226111 @default.
- W2038022611 hasRelatedWork W1909456581 @default.
- W2038022611 hasRelatedWork W1995238134 @default.
- W2038022611 hasRelatedWork W2010247437 @default.
- W2038022611 hasRelatedWork W2022916846 @default.
- W2038022611 hasRelatedWork W2033329810 @default.
- W2038022611 hasRelatedWork W2037432273 @default.
- W2038022611 hasRelatedWork W2360555823 @default.
- W2038022611 hasRelatedWork W2754382840 @default.
- W2038022611 hasRelatedWork W2990169890 @default.
- W2038022611 hasRelatedWork W3030061171 @default.
- W2038022611 isParatext "false" @default.
- W2038022611 isRetracted "false" @default.
- W2038022611 magId "2038022611" @default.
- W2038022611 workType "article" @default.