Matches in SemOpenAlex for { <https://semopenalex.org/work/W2038041346> ?p ?o ?g. }
- W2038041346 endingPage "266" @default.
- W2038041346 startingPage "253" @default.
- W2038041346 abstract "The effects of glutamate and other more selective excitatory amino acid (EAA) analogs on intracellular free calcium concentration ( [Ca2+]i) were examined in Fura 2-loaded cultured chick embryo cortical cells (90% neuronal). Four EAA receptors were evident in these studies: an N-methyl-D-aspartate (NMDA) receptor, a kainate receptor, and two quisqualate receptors. The [Ca2+]i response to NMDA was blocked or reversed by selective antagonists such as 2-amino-5-phosphonovalerate (APV), MK801 and ketamine, as well as by desmethylimipramine and dextromethorphan. Glycine potentiated the [Ca2+]i response to NMDA, and high concentrations of glycine selectively overcame blockade by kynurenic acid, 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX), and cis-piperidine-2,3-dicarboxylic acid (PDA). The [(Ca2+]i response to kainate was generally larger than the NMDA response, and the kainate response desensitized slightly over the first minute. CNQX was more potent as an antagonist of the kainate response than of the NMDA response, even in the absence of added glycine; kynurenic acid and PDA conversely had little effect on the kainate response in these cells at concentrations which blocked the NMDA response. The desensitization of the [Ca2+]i response to kainate was greatly augmented by quisqualate and by the putative ionotropic quisqualate receptor agonist alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA). In the absence of kainate, both quisqualate and AMPA increased [Ca2+]i though less so than did NMDA or kainate. Quisqualate (and AMPA and glutamate) were not acting as partial agonists at the kainate receptor, since the potency of these agonists in reversing the kainate [Ca2+]i response was independent of kainate concentration. Quisqualate, but not AMPA, also produced a small increase in [Ca2+]i which preceded the negative effect of this agonist on the kainate response. This increase in [Ca2+]i could also be evoked by quisqualate or glutamate after inhibition of the kainate response by AMPA. Quisqualate and glutamate, but not the other EAA agonists, also increased [Ca2+]i after chelation of extracellular calcium with EGTA. This effect appears to be mediated by the metabotropic quisqualate receptor. These cells should provide a useful system for studying regulation and interactions of EAA receptors, and for screening drugs which might act at these receptors." @default.
- W2038041346 created "2016-06-24" @default.
- W2038041346 creator A5029857264 @default.
- W2038041346 creator A5063433642 @default.
- W2038041346 creator A5072778588 @default.
- W2038041346 date "1990-10-01" @default.
- W2038041346 modified "2023-09-27" @default.
- W2038041346 title "Characterization of Ca2+-mobilizing excitatory amino acid receptors in cultured chick cortical cells" @default.
- W2038041346 cites W1487954084 @default.
- W2038041346 cites W1511237228 @default.
- W2038041346 cites W1564137887 @default.
- W2038041346 cites W1608531923 @default.
- W2038041346 cites W1967113275 @default.
- W2038041346 cites W1971547182 @default.
- W2038041346 cites W1973382533 @default.
- W2038041346 cites W1975342713 @default.
- W2038041346 cites W1983378730 @default.
- W2038041346 cites W1985053181 @default.
- W2038041346 cites W1987350345 @default.
- W2038041346 cites W1998950113 @default.
- W2038041346 cites W2003444707 @default.
- W2038041346 cites W2005711621 @default.
- W2038041346 cites W2010713665 @default.
- W2038041346 cites W2017439663 @default.
- W2038041346 cites W2025114609 @default.
- W2038041346 cites W2028405447 @default.
- W2038041346 cites W2029313413 @default.
- W2038041346 cites W2037772094 @default.
- W2038041346 cites W2062149063 @default.
- W2038041346 cites W2062390007 @default.
- W2038041346 cites W2067342671 @default.
- W2038041346 cites W2087069075 @default.
- W2038041346 cites W2093232343 @default.
- W2038041346 cites W2096535756 @default.
- W2038041346 cites W2097057862 @default.
- W2038041346 cites W2100040827 @default.
- W2038041346 cites W2110868914 @default.
- W2038041346 cites W2121624942 @default.
- W2038041346 cites W2127725525 @default.
- W2038041346 cites W2137376734 @default.
- W2038041346 cites W2170029299 @default.
- W2038041346 cites W2180941170 @default.
- W2038041346 doi "https://doi.org/10.1016/0922-4106(90)90118-h" @default.
- W2038041346 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1980647" @default.
- W2038041346 hasPublicationYear "1990" @default.
- W2038041346 type Work @default.
- W2038041346 sameAs 2038041346 @default.
- W2038041346 citedByCount "11" @default.
- W2038041346 crossrefType "journal-article" @default.
- W2038041346 hasAuthorship W2038041346A5029857264 @default.
- W2038041346 hasAuthorship W2038041346A5063433642 @default.
- W2038041346 hasAuthorship W2038041346A5072778588 @default.
- W2038041346 hasConcept C112592302 @default.
- W2038041346 hasConcept C126322002 @default.
- W2038041346 hasConcept C160268369 @default.
- W2038041346 hasConcept C170493617 @default.
- W2038041346 hasConcept C185592680 @default.
- W2038041346 hasConcept C189184402 @default.
- W2038041346 hasConcept C2776841986 @default.
- W2038041346 hasConcept C2778938600 @default.
- W2038041346 hasConcept C2779803574 @default.
- W2038041346 hasConcept C55493867 @default.
- W2038041346 hasConcept C61174792 @default.
- W2038041346 hasConcept C67018056 @default.
- W2038041346 hasConcept C71924100 @default.
- W2038041346 hasConcept C73009533 @default.
- W2038041346 hasConcept C86803240 @default.
- W2038041346 hasConceptScore W2038041346C112592302 @default.
- W2038041346 hasConceptScore W2038041346C126322002 @default.
- W2038041346 hasConceptScore W2038041346C160268369 @default.
- W2038041346 hasConceptScore W2038041346C170493617 @default.
- W2038041346 hasConceptScore W2038041346C185592680 @default.
- W2038041346 hasConceptScore W2038041346C189184402 @default.
- W2038041346 hasConceptScore W2038041346C2776841986 @default.
- W2038041346 hasConceptScore W2038041346C2778938600 @default.
- W2038041346 hasConceptScore W2038041346C2779803574 @default.
- W2038041346 hasConceptScore W2038041346C55493867 @default.
- W2038041346 hasConceptScore W2038041346C61174792 @default.
- W2038041346 hasConceptScore W2038041346C67018056 @default.
- W2038041346 hasConceptScore W2038041346C71924100 @default.
- W2038041346 hasConceptScore W2038041346C73009533 @default.
- W2038041346 hasConceptScore W2038041346C86803240 @default.
- W2038041346 hasIssue "4-5" @default.
- W2038041346 hasLocation W20380413461 @default.
- W2038041346 hasLocation W20380413462 @default.
- W2038041346 hasOpenAccess W2038041346 @default.
- W2038041346 hasPrimaryLocation W20380413461 @default.
- W2038041346 hasRelatedWork W112497821 @default.
- W2038041346 hasRelatedWork W1916309944 @default.
- W2038041346 hasRelatedWork W1987264077 @default.
- W2038041346 hasRelatedWork W2029403919 @default.
- W2038041346 hasRelatedWork W2038041346 @default.
- W2038041346 hasRelatedWork W2041644100 @default.
- W2038041346 hasRelatedWork W2212696258 @default.
- W2038041346 hasRelatedWork W2474607999 @default.
- W2038041346 hasRelatedWork W2753475615 @default.
- W2038041346 hasRelatedWork W2316593469 @default.
- W2038041346 hasVolume "189" @default.