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- W2038049392 abstract "The process of cell fusion of Ehrlich ascites tumor cells by a high concentration of HVJ virions in vitro was studied by electron microscopy to clarify the role of the virions in cell fusion. The study showed that the cell fusion proceeds along the following morphologically distinct steps: (1) agglutination of cells by virions in the cold; (2) close contact of adjacent cell membranes near adsorbed virions of agglutinated cells; (3) degradation of the cell membranes in contact with virions and communication of the cytoplasms of adjacent cells through the breaks; (4) connection of broken cell membranes between adjacent cells and formation of cytoplasmic bridges; (5) enlargement of the bridges and engulfing of virions within vesicles; (6) rounding of fused cells and alteration of the virions within vesicles, followed by the appearance of free nucleocapsids in cytoplasm. Steps 1 and 3 depend directly on the virions; the virions agglutinate cells in step 1 and have a degrading effect on cell membranes in step 3. These results imply that the site of cytoplasmic bridges is determined by adsorbed virions and that the phagocytosis of virions occurs later than formation of the bridges." @default.
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- W2038049392 date "1968-03-01" @default.
- W2038049392 modified "2023-09-27" @default.
- W2038049392 title "Electron microscopic study of cell fusion by HVJ virions" @default.
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- W2038049392 doi "https://doi.org/10.1016/0042-6822(68)90062-7" @default.
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