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• W2038049850 abstract "Removal of low levels (0.3–1.3 μM) of primary amines (PA) were examined by direct-injection liquid chromatography in offshore Lake Michigan water to provide insight about potential cycling rates of labile organic compounds and to examine the hypothesis that populations of active bacteria are controlled by organic-substrate limitation. Uptake rates of amino acids in untreated lake water ranged from <0.1 to 3.7 nmole PA L−1h−1 (0.01 to 0.34 μg C L−1h−1) as compared to rates of up to 9 nmole PA L−1h−1 (0.61 μg C L−1h−1) in samples fortified with amino acids. Highest PA uptake rates were observed in samples collected in July and August. As expected for Michaelis-Menton kinetics, PA uptake rates increased with increasing amino acid concentrations (up to a maximum velocity), suggesting that bacterial “growth rates” were substrate limited. However, PA removal rates in given lake water samples usually remained relatively constant over periods of several days. The consistency of population uptake rates over time suggested that bacterial abundances did not change greatly in response to amino acid additions. Thus, although bacterial growth rates in Lake Michigan epilimnetic water are apparently controlled by organic substrate supply, our data are consistent with the hypothesis that bacterial abundances, and therefore total-population heterotrophic uptake activity, may often be controlled by grazers. The chemical approach used in this study yields a conservative estimate of the maximum velocity of microbial use of amino acids in natural waters." @default.
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• W2038049850 date "1986-01-01" @default.
• W2038049850 modified "2023-09-25" @default.
• W2038049850 title "Microbial Response to Amino Acid Additions in Lake Michigan: Grazer Control and Substrate Limitation of Bacterial Populations" @default.
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• W2038049850 doi "https://doi.org/10.1016/s0380-1330(86)71715-2" @default.
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