FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2038051211> ?p ?o ?g. }

• W2038051211 endingPage "13146" @default.
• W2038051211 startingPage "13138" @default.
• W2038051211 abstract "Protein farnesyltransferase (PFTase) is a zinc metalloenzyme that catalyzes the posttranslational alkylation of the cysteine in C-terminal -Ca(1)a(2)X sequences by a 15-carbon farnesyl residue, where C is cysteine, a(1) and a(2) are normally aliphatic amino acids, and X is an amino acid that specifies selectivity for the farnesyl moiety. Formation of a Zn(2+) thiolate in the PFTase. peptide complex was detected by the appearance of an absorbance at 236 nm (epsilon = 15 000 M(-1) cm(-1)), which was dependent on the concentration of peptide, in a UV difference spectrum in a solution of PFTase and the peptide substrate RTRCVIA. We developed a fluorescence anisotropy binding assay to measure the dissociation constants as a function of pH for peptide analogues by appending a 2',7'-difluorofluorescein to their N-terminus. The electron-withdrawing fluorine atoms allowed us to measure peptide binding down to pH 5.5 without having to correct for the changes in fluorescence intensity that accompany protonation of the fluorophore. Measurements of the pK(a)s for thiol groups in free and bound peptide indicate that peptide binding is accompanied by formation of a zinc thiolate and that binding to PFTase lowers the pK of the peptide thiol by 3 units. In similar studies with the betaY310F mutant, the pK(a) of the thiol moiety was lowered by 2 units upon binding, indicating that the hydroxyl group in the conserved tyrosine helps stabilize the bound thiolate." @default.
• W2038051211 created "2016-06-24" @default.
• W2038051211 creator A5067280611 @default.
• W2038051211 creator A5075589364 @default.
• W2038051211 date "1999-09-10" @default.
• W2038051211 modified "2023-09-27" @default.
• W2038051211 title "Yeast Protein Farnesyltransferase. p<i>K</i>_as of Peptide Substrates Bound as Zinc Thiolates" @default.
• W2038051211 cites W1482012198 @default.
• W2038051211 cites W1483337170 @default.
• W2038051211 cites W1505669099 @default.
• W2038051211 cites W1507719842 @default.
• W2038051211 cites W1522846126 @default.
• W2038051211 cites W1557478309 @default.
• W2038051211 cites W1589687205 @default.
• W2038051211 cites W1602721680 @default.
• W2038051211 cites W1972154782 @default.
• W2038051211 cites W1979103455 @default.
• W2038051211 cites W1984134606 @default.
• W2038051211 cites W1985410055 @default.
• W2038051211 cites W1985467496 @default.
• W2038051211 cites W1988463607 @default.
• W2038051211 cites W1989016417 @default.
• W2038051211 cites W1993418815 @default.
• W2038051211 cites W1993495402 @default.
• W2038051211 cites W1999323300 @default.
• W2038051211 cites W2002986759 @default.
• W2038051211 cites W2008998644 @default.
• W2038051211 cites W2011529244 @default.
• W2038051211 cites W2024166317 @default.
• W2038051211 cites W2026834760 @default.
• W2038051211 cites W2038556246 @default.
• W2038051211 cites W2038825773 @default.
• W2038051211 cites W2041188540 @default.
• W2038051211 cites W2056942156 @default.
• W2038051211 cites W2060264934 @default.
• W2038051211 cites W2060531375 @default.
• W2038051211 cites W2065782985 @default.
• W2038051211 cites W2068778863 @default.
• W2038051211 cites W2075079950 @default.
• W2038051211 cites W2082080415 @default.
• W2038051211 cites W2084972848 @default.
• W2038051211 cites W2090487035 @default.
• W2038051211 cites W2090702802 @default.
• W2038051211 cites W2093553051 @default.
• W2038051211 cites W2093813824 @default.
• W2038051211 cites W2098806204 @default.
• W2038051211 cites W2176744910 @default.
• W2038051211 cites W2200991233 @default.
• W2038051211 cites W2231483212 @default.
• W2038051211 cites W2950523840 @default.
• W2038051211 cites W2951404851 @default.
• W2038051211 cites W2953153819 @default.
• W2038051211 cites W650972495 @default.
• W2038051211 doi "https://doi.org/10.1021/bi990794y" @default.
• W2038051211 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10529185" @default.
• W2038051211 hasPublicationYear "1999" @default.
• W2038051211 type Work @default.
• W2038051211 sameAs 2038051211 @default.
• W2038051211 citedByCount "31" @default.
• W2038051211 countsByYear W20380512112013 @default.
• W2038051211 countsByYear W20380512112014 @default.
• W2038051211 countsByYear W20380512112016 @default.
• W2038051211 countsByYear W20380512112017 @default.
• W2038051211 countsByYear W20380512112018 @default.
• W2038051211 countsByYear W20380512112021 @default.
• W2038051211 crossrefType "journal-article" @default.
• W2038051211 hasAuthorship W2038051211A5067280611 @default.
• W2038051211 hasAuthorship W2038051211A5075589364 @default.
• W2038051211 hasConcept C145148216 @default.
• W2038051211 hasConcept C178790620 @default.
• W2038051211 hasConcept C181199279 @default.
• W2038051211 hasConcept C185592680 @default.
• W2038051211 hasConcept C2776568683 @default.
• W2038051211 hasConcept C2778396349 @default.
• W2038051211 hasConcept C2779201268 @default.
• W2038051211 hasConcept C2779281246 @default.
• W2038051211 hasConcept C2781324293 @default.
• W2038051211 hasConcept C30095370 @default.
• W2038051211 hasConcept C55493867 @default.
• W2038051211 hasConcept C57790582 @default.
• W2038051211 hasConcept C71240020 @default.
• W2038051211 hasConceptScore W2038051211C145148216 @default.
• W2038051211 hasConceptScore W2038051211C178790620 @default.
• W2038051211 hasConceptScore W2038051211C181199279 @default.
• W2038051211 hasConceptScore W2038051211C185592680 @default.
• W2038051211 hasConceptScore W2038051211C2776568683 @default.
• W2038051211 hasConceptScore W2038051211C2778396349 @default.
• W2038051211 hasConceptScore W2038051211C2779201268 @default.
• W2038051211 hasConceptScore W2038051211C2779281246 @default.
• W2038051211 hasConceptScore W2038051211C2781324293 @default.
• W2038051211 hasConceptScore W2038051211C30095370 @default.
• W2038051211 hasConceptScore W2038051211C55493867 @default.
• W2038051211 hasConceptScore W2038051211C57790582 @default.
• W2038051211 hasConceptScore W2038051211C71240020 @default.
• W2038051211 hasIssue "40" @default.
• W2038051211 hasLocation W20380512111 @default.
• W2038051211 hasLocation W20380512112 @default.
• W2038051211 hasOpenAccess W2038051211 @default.

• next