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• W2038091408 abstract "RNA is extracted from purified plastids isolated from young leaves of spinach and is electrophoresed in fully denaturing conditions with 98% of formamide in the gels. The 16 S and 23 S rRNA of Escherichia coli were used as internal standards. The plastid 16 S rRNA is made of unbroken polynucleotide chains (Mr = 0.535 ± 0.005 · 106; the value of 0.54 · 106 is used). Some of the molecules of the 23 S rRNA are also unbroken polynucleotide chains of 1.28 ± 0.03 · 106 daltons while the others are composed of several fragments connected together by hydrogen bonding. Some of these fragments are detected in large relative amounts with the following Mr : 0.71 ± 0.01 · 106; 0.43 ± 0.01 · 106; 0.35 ± 0.01 · 106 and 0.15 ± 0.01 · 106; others are detected in low relative amounts with the following Mr: 0.98 ± 0.03 · 106 and 0.27 ± 0.02 · 106. All these fragments results from the denaturation in formamide of the nicked (post-matured) 23 S molecules. The determination of Mr of each species has been done with isolated molecules of 23 S and 16 S rRNA. The Mr of p23 S and of p16 S are respectively 1.54 ± 0.03 · 106 and 0.7 · 106. The newly synthesized 23 S molecules have a Mr of 1.28 ± 0.03 · 106 (unbroken polynucleotide chain). The post-maturation of the 23 S is demonstrated by a pulse-chase experiment." @default.
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• W2038091408 date "1978-02-01" @default.
• W2038091408 modified "2023-09-27" @default.
• W2038091408 title "Molecular weight determination of precursor, mature and post-mature plastid ribosomal RNA from spinach using fully denaturing conditions" @default.
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• W2038091408 doi "https://doi.org/10.1016/0005-2787(78)90205-8" @default.
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