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• W2038092857 abstract "A new technique has been developed for the isolation of membrane vesicles from the vitamin D-deficient and vitamin D-treated chick intestinal brush border membrane. The technique involves removal of nuclei from a low speed pellet by discontinuous sucrose gradient centrifugation. The resulting intact brush borders are then homogenized in 0.5 M Tris and the membrane fragments purified on a glycerol gradient. This preparation represents a 20-fold purification of the brush border marker sucrase. After 1α-hydroxyvitamin D3 treatment there is a significant increase in membrane phospholipid phosphorous, an alteration in the fatty acid composition of the phosphatidylcholine fraction of membrane phospholipid, and a decrease in sucrase specific activity." @default.
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• W2038092857 date "1978-08-01" @default.
• W2038092857 modified "2023-10-16" @default.
• W2038092857 title "Purification and characterization of chick intestine brush border membrane Effects of 1α(OH) vitamin D3 treatment" @default.
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• W2038092857 doi "https://doi.org/10.1016/0005-2736(78)90316-4" @default.
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