Matches in SemOpenAlex for { <https://semopenalex.org/work/W2038098052> ?p ?o ?g. }
- W2038098052 endingPage "4803" @default.
- W2038098052 startingPage "4797" @default.
- W2038098052 abstract "Hepatic microsomes isolated from untreated male rats or from rats pretreated with phenobarbital (PB) or 3-methylcholanthrene (3-MC) were labeled with the hydrophobic, photoactivated reagent 3-(trifluoromethyl)-3-(m-[125I]iodophenyl)diazirine ([125I]TID). [125I]TID incorporation into 3-MC- and PB-induced liver microsomal protein was enhanced 5- and 8-fold, respectively, relative to the incorporation of [125I]TID into uninduced liver microsomes. The major hepatic microsomal cytochrome P-450 forms inducible by PB and 3-MC, respectively designated P-450s PB-4 and BNF-B, were shown to be the principal polypeptides labeled by [125I]TID in the correspondingly induced microsomes. Trypsin cleavage of [125I]TID-labeled microsomal P-450 PB-4 yielded several radiolabeled fragments, with a single labeled peptide of Mr approximately 4000 resistant to extensive proteolytic digestion. The following experiments suggested that TID binds to the substrate-binding site of P-450 PB-4. [125I]TID incorporation into microsomal P-450 PB-4 was inhibited in a dose-dependent manner by the P-450 PB-4 substrate benzphetamine. In the absence of photoactivation, TID inhibited competitively about 80% of the cytochrome P-450-dependent 7-ethoxycoumarin O-deethylation catalyzed by PB-induced microsomes with a Ki of 10 microM; TID was a markedly less effective inhibitor of the corresponding activity catalyzed by microsomes isolated from uninduced or beta-naphthoflavone-induced livers.(ABSTRACT TRUNCATED AT 250 WORDS)" @default.
- W2038098052 created "2016-06-24" @default.
- W2038098052 creator A5009700420 @default.
- W2038098052 creator A5044985980 @default.
- W2038098052 creator A5048051211 @default.
- W2038098052 creator A5065416200 @default.
- W2038098052 creator A5080761072 @default.
- W2038098052 date "1986-08-26" @default.
- W2038098052 modified "2023-09-25" @default.
- W2038098052 title "3-(Trifluoromethyl)-3-(m-[125I]iodophenyl)diazirine photolabels a substrate-binding site of rat hepatic cytochrome P-450 form PB-4" @default.
- W2038098052 cites W126980359 @default.
- W2038098052 cites W1481352963 @default.
- W2038098052 cites W1502053909 @default.
- W2038098052 cites W1503159803 @default.
- W2038098052 cites W1522770871 @default.
- W2038098052 cites W1530231515 @default.
- W2038098052 cites W1540602405 @default.
- W2038098052 cites W1541048010 @default.
- W2038098052 cites W1552750745 @default.
- W2038098052 cites W1584235297 @default.
- W2038098052 cites W1590889826 @default.
- W2038098052 cites W1592104282 @default.
- W2038098052 cites W1594851571 @default.
- W2038098052 cites W1633747844 @default.
- W2038098052 cites W1722287580 @default.
- W2038098052 cites W1728002571 @default.
- W2038098052 cites W1775749144 @default.
- W2038098052 cites W186679341 @default.
- W2038098052 cites W1885778179 @default.
- W2038098052 cites W1917764047 @default.
- W2038098052 cites W1959524523 @default.
- W2038098052 cites W1971023121 @default.
- W2038098052 cites W1975032872 @default.
- W2038098052 cites W1981435376 @default.
- W2038098052 cites W2007948620 @default.
- W2038098052 cites W2010207116 @default.
- W2038098052 cites W2014632368 @default.
- W2038098052 cites W2022628424 @default.
- W2038098052 cites W2025826147 @default.
- W2038098052 cites W2028581995 @default.
- W2038098052 cites W2031423487 @default.
- W2038098052 cites W2064497693 @default.
- W2038098052 cites W2071219823 @default.
- W2038098052 cites W2075374803 @default.
- W2038098052 cites W2076985212 @default.
- W2038098052 cites W2086106892 @default.
- W2038098052 cites W2086295189 @default.
- W2038098052 cites W2100837269 @default.
- W2038098052 cites W2132254282 @default.
- W2038098052 cites W2151201559 @default.
- W2038098052 cites W2169838870 @default.
- W2038098052 cites W2182056816 @default.
- W2038098052 cites W4302566315 @default.
- W2038098052 doi "https://doi.org/10.1021/bi00365a012" @default.
- W2038098052 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3768313" @default.
- W2038098052 hasPublicationYear "1986" @default.
- W2038098052 type Work @default.
- W2038098052 sameAs 2038098052 @default.
- W2038098052 citedByCount "20" @default.
- W2038098052 crossrefType "journal-article" @default.
- W2038098052 hasAuthorship W2038098052A5009700420 @default.
- W2038098052 hasAuthorship W2038098052A5044985980 @default.
- W2038098052 hasAuthorship W2038098052A5048051211 @default.
- W2038098052 hasAuthorship W2038098052A5065416200 @default.
- W2038098052 hasAuthorship W2038098052A5080761072 @default.
- W2038098052 hasConcept C153911025 @default.
- W2038098052 hasConcept C178790620 @default.
- W2038098052 hasConcept C181199279 @default.
- W2038098052 hasConcept C185592680 @default.
- W2038098052 hasConcept C18903297 @default.
- W2038098052 hasConcept C2776442012 @default.
- W2038098052 hasConcept C2777289219 @default.
- W2038098052 hasConcept C2777606763 @default.
- W2038098052 hasConcept C2779281246 @default.
- W2038098052 hasConcept C2780263894 @default.
- W2038098052 hasConcept C2780340462 @default.
- W2038098052 hasConcept C2780768313 @default.
- W2038098052 hasConcept C2780815831 @default.
- W2038098052 hasConcept C55493867 @default.
- W2038098052 hasConcept C71240020 @default.
- W2038098052 hasConcept C86803240 @default.
- W2038098052 hasConcept C87644729 @default.
- W2038098052 hasConceptScore W2038098052C153911025 @default.
- W2038098052 hasConceptScore W2038098052C178790620 @default.
- W2038098052 hasConceptScore W2038098052C181199279 @default.
- W2038098052 hasConceptScore W2038098052C185592680 @default.
- W2038098052 hasConceptScore W2038098052C18903297 @default.
- W2038098052 hasConceptScore W2038098052C2776442012 @default.
- W2038098052 hasConceptScore W2038098052C2777289219 @default.
- W2038098052 hasConceptScore W2038098052C2777606763 @default.
- W2038098052 hasConceptScore W2038098052C2779281246 @default.
- W2038098052 hasConceptScore W2038098052C2780263894 @default.
- W2038098052 hasConceptScore W2038098052C2780340462 @default.
- W2038098052 hasConceptScore W2038098052C2780768313 @default.
- W2038098052 hasConceptScore W2038098052C2780815831 @default.
- W2038098052 hasConceptScore W2038098052C55493867 @default.
- W2038098052 hasConceptScore W2038098052C71240020 @default.
- W2038098052 hasConceptScore W2038098052C86803240 @default.