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- W2038103688 abstract "HIV-1 reverse transcriptase is a dimeric enzyme mainly Involved in the replication of the viral genome. A filamentous phage cDNA expression library from human lymphocytes was used to select cellular proteins interacting with HIV-1 reverse transcriptase. Affinity selections using the bacterially expressed monomeric large subunit of reverse transcriptase (p66) yielded host β-actin. This clone was expressed as glutathione-S-transferase fusion protein which was identified by using a specific antibody against β-actin. Furthermore we show that also the eukaryotic β-actin binds to either the large subunit of reverse transcriptase or to the Pol precursor polyprotein in vitro . The reverse transcriptase/β-actin interaction might be important for the secretion of HIV-1 virions." @default.
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- W2038103688 date "1995-01-01" @default.
- W2038103688 modified "2023-09-26" @default.
- W2038103688 title "The large subunit of HIV-1 reverse transcriptase interacts with β-actin" @default.
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- W2038103688 doi "https://doi.org/10.1093/nar/23.5.736" @default.
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