Matches in SemOpenAlex for { <https://semopenalex.org/work/W2038183281> ?p ?o ?g. }
Showing items 1 to 86 of
86
with 100 items per page.
- W2038183281 endingPage "1133" @default.
- W2038183281 startingPage "1129" @default.
- W2038183281 abstract "We have developed an oil- microcentrifuge assay system for analyzing the binding of [3H]estradiol in metabolically active MCF-7 and MDA human breast cancer cells. Complete separation of 2 X 10(6) cells from radioactive media can be achieved within 5 s of centrifugation at 12,000 rpm. The [3H]estradiol binding sites in MCF-7 cells are filled within 20 min of radioligand exposure. Using this assay, our MCF-7 cells contain approximately 15,000 high affinity and saturable binding sites. Binding is inhibited by estradiol and tamoxifen but not progesterone. There is no specific binding of [3H]estradiol in MDA cells. This assay is a rapid, sensitive and reproducible method for investigating hormone-receptor binding and ligand specificity in cultured cells; results compare favorably with those obtained by more complex and lengthy techniques." @default.
- W2038183281 created "2016-06-24" @default.
- W2038183281 creator A5023160549 @default.
- W2038183281 creator A5062040702 @default.
- W2038183281 creator A5063981448 @default.
- W2038183281 creator A5083125470 @default.
- W2038183281 creator A5085770790 @default.
- W2038183281 date "1984-05-01" @default.
- W2038183281 modified "2023-10-16" @default.
- W2038183281 title "The oil-microcentrifuge assay: A rapid and sensitive method for analyzing specific [3H]estradiol binding in cultured cells" @default.
- W2038183281 cites W1599043290 @default.
- W2038183281 cites W1969337131 @default.
- W2038183281 cites W1996282495 @default.
- W2038183281 cites W2006692219 @default.
- W2038183281 cites W2025102444 @default.
- W2038183281 cites W2062338639 @default.
- W2038183281 cites W2070657519 @default.
- W2038183281 cites W2075536135 @default.
- W2038183281 cites W2103885797 @default.
- W2038183281 cites W2255395527 @default.
- W2038183281 doi "https://doi.org/10.1016/0022-4731(84)90355-8" @default.
- W2038183281 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6727363" @default.
- W2038183281 hasPublicationYear "1984" @default.
- W2038183281 type Work @default.
- W2038183281 sameAs 2038183281 @default.
- W2038183281 citedByCount "14" @default.
- W2038183281 crossrefType "journal-article" @default.
- W2038183281 hasAuthorship W2038183281A5023160549 @default.
- W2038183281 hasAuthorship W2038183281A5062040702 @default.
- W2038183281 hasAuthorship W2038183281A5063981448 @default.
- W2038183281 hasAuthorship W2038183281A5083125470 @default.
- W2038183281 hasAuthorship W2038183281A5085770790 @default.
- W2038183281 hasConcept C116569031 @default.
- W2038183281 hasConcept C121608353 @default.
- W2038183281 hasConcept C153911025 @default.
- W2038183281 hasConcept C170493617 @default.
- W2038183281 hasConcept C185592680 @default.
- W2038183281 hasConcept C2777176818 @default.
- W2038183281 hasConcept C2909005528 @default.
- W2038183281 hasConcept C43617362 @default.
- W2038183281 hasConcept C530470458 @default.
- W2038183281 hasConcept C54355233 @default.
- W2038183281 hasConcept C55493867 @default.
- W2038183281 hasConcept C67847695 @default.
- W2038183281 hasConcept C7623868 @default.
- W2038183281 hasConcept C84606932 @default.
- W2038183281 hasConcept C85196483 @default.
- W2038183281 hasConcept C86803240 @default.
- W2038183281 hasConceptScore W2038183281C116569031 @default.
- W2038183281 hasConceptScore W2038183281C121608353 @default.
- W2038183281 hasConceptScore W2038183281C153911025 @default.
- W2038183281 hasConceptScore W2038183281C170493617 @default.
- W2038183281 hasConceptScore W2038183281C185592680 @default.
- W2038183281 hasConceptScore W2038183281C2777176818 @default.
- W2038183281 hasConceptScore W2038183281C2909005528 @default.
- W2038183281 hasConceptScore W2038183281C43617362 @default.
- W2038183281 hasConceptScore W2038183281C530470458 @default.
- W2038183281 hasConceptScore W2038183281C54355233 @default.
- W2038183281 hasConceptScore W2038183281C55493867 @default.
- W2038183281 hasConceptScore W2038183281C67847695 @default.
- W2038183281 hasConceptScore W2038183281C7623868 @default.
- W2038183281 hasConceptScore W2038183281C84606932 @default.
- W2038183281 hasConceptScore W2038183281C85196483 @default.
- W2038183281 hasConceptScore W2038183281C86803240 @default.
- W2038183281 hasIssue "5" @default.
- W2038183281 hasLocation W20381832811 @default.
- W2038183281 hasLocation W20381832812 @default.
- W2038183281 hasOpenAccess W2038183281 @default.
- W2038183281 hasPrimaryLocation W20381832811 @default.
- W2038183281 hasRelatedWork W100235375 @default.
- W2038183281 hasRelatedWork W1828383458 @default.
- W2038183281 hasRelatedWork W1897131549 @default.
- W2038183281 hasRelatedWork W1985444891 @default.
- W2038183281 hasRelatedWork W2007930722 @default.
- W2038183281 hasRelatedWork W2037645853 @default.
- W2038183281 hasRelatedWork W2178161815 @default.
- W2038183281 hasRelatedWork W2312486636 @default.
- W2038183281 hasRelatedWork W2417921410 @default.
- W2038183281 hasRelatedWork W2978892524 @default.
- W2038183281 hasVolume "20" @default.
- W2038183281 isParatext "false" @default.
- W2038183281 isRetracted "false" @default.
- W2038183281 magId "2038183281" @default.
- W2038183281 workType "article" @default.