FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2038230871> ?p ?o ?g. }

• W2038230871 endingPage "224" @default.
• W2038230871 startingPage "212" @default.
• W2038230871 abstract "The spike glycoprotein of mouse hepatitis virus strain A59 mediates the early events leading to infection of cells, including fusion of the viral and cellular membranes. The spike is a type I membrane glycoprotein that possesses a conserved transmembrane anchor and an unusual cysteine-rich (cys) domain that bridges the putative junction of the anchor and the cytoplasmic tail. In this study, we examined the role of these carboxyl-terminal domains in spike-mediated membrane fusion. We show that the cytoplasmic tail is not required for fusion but has the capacity to enhance membrane fusion activity. Chimeric spike protein mutants containing substitutions of the entire transmembrane anchor and cys domain with the herpes simplex virus type 1 glycoprotein D (gD-1) anchor demonstrated that fusion activity requires the presence of the A59 membrane-spanning domain and the portion of the cys domain that lies upstream of the cytoplasmic tail. The cys domain is a required element since its deletion from the wild-type spike protein abrogates fusion activity. However, addition of the cys domain to fusion-defective chimeric proteins was unable to restore fusion activity. Thus, the cys domain is necessary but is not sufficient to complement the gD-1 anchor and allow for membrane fusion. Site-specific mutations of conserved cysteine residues in the cys domain markedly reduce membrane fusion, which further supports the conclusion that this region is crucial for spike function. The results indicate that the carboxyl-terminus of the spike transmembrane anchor contains at least two distinct domains, both of which are necessary for full membrane fusion." @default.
• W2038230871 created "2016-06-24" @default.
• W2038230871 creator A5000975940 @default.
• W2038230871 creator A5025152973 @default.
• W2038230871 creator A5062291725 @default.
• W2038230871 date "2000-03-01" @default.
• W2038230871 modified "2023-09-27" @default.
• W2038230871 title "Coronavirus-Induced Membrane Fusion Requires the Cysteine-Rich Domain in the Spike Protein" @default.
• W2038230871 cites W130998851 @default.
• W2038230871 cites W1487090760 @default.
• W2038230871 cites W1505738988 @default.
• W2038230871 cites W1529440054 @default.
• W2038230871 cites W1545952535 @default.
• W2038230871 cites W1556716938 @default.
• W2038230871 cites W1582112214 @default.
• W2038230871 cites W1595120220 @default.
• W2038230871 cites W1602267385 @default.
• W2038230871 cites W1760105922 @default.
• W2038230871 cites W1760183277 @default.
• W2038230871 cites W1837543172 @default.
• W2038230871 cites W1860579418 @default.
• W2038230871 cites W1880482445 @default.
• W2038230871 cites W1896763970 @default.
• W2038230871 cites W1931522421 @default.
• W2038230871 cites W1943421922 @default.
• W2038230871 cites W1957495804 @default.
• W2038230871 cites W1959122025 @default.
• W2038230871 cites W1963865659 @default.
• W2038230871 cites W1965262656 @default.
• W2038230871 cites W1968563729 @default.
• W2038230871 cites W1974967271 @default.
• W2038230871 cites W1978044705 @default.
• W2038230871 cites W1979615660 @default.
• W2038230871 cites W1983926699 @default.
• W2038230871 cites W1988762133 @default.
• W2038230871 cites W1991032901 @default.
• W2038230871 cites W1995189663 @default.
• W2038230871 cites W1997482909 @default.
• W2038230871 cites W2001500248 @default.
• W2038230871 cites W2005411001 @default.
• W2038230871 cites W2005949226 @default.
• W2038230871 cites W2015179413 @default.
• W2038230871 cites W2019723399 @default.
• W2038230871 cites W2021122318 @default.
• W2038230871 cites W2024764884 @default.
• W2038230871 cites W2026088739 @default.
• W2038230871 cites W2037132984 @default.
• W2038230871 cites W2039278431 @default.
• W2038230871 cites W2043355913 @default.
• W2038230871 cites W2051566412 @default.
• W2038230871 cites W2056541157 @default.
• W2038230871 cites W2057284222 @default.
• W2038230871 cites W2067797438 @default.
• W2038230871 cites W2068673200 @default.
• W2038230871 cites W2069731701 @default.
• W2038230871 cites W2074769546 @default.
• W2038230871 cites W2076462632 @default.
• W2038230871 cites W2087363345 @default.
• W2038230871 cites W2094413071 @default.
• W2038230871 cites W2096700474 @default.
• W2038230871 cites W2108256479 @default.
• W2038230871 cites W2114653876 @default.
• W2038230871 cites W2117170332 @default.
• W2038230871 cites W2117969454 @default.
• W2038230871 cites W2123852904 @default.
• W2038230871 cites W2131071571 @default.
• W2038230871 cites W2131505335 @default.
• W2038230871 cites W2132422301 @default.
• W2038230871 cites W2139090502 @default.
• W2038230871 cites W2141162592 @default.
• W2038230871 cites W2147755019 @default.
• W2038230871 cites W2152912823 @default.
• W2038230871 cites W2160029608 @default.
• W2038230871 cites W2160809286 @default.
• W2038230871 cites W2162183496 @default.
• W2038230871 cites W2166292354 @default.
• W2038230871 cites W2166668702 @default.
• W2038230871 cites W2168848638 @default.
• W2038230871 cites W2169951117 @default.
• W2038230871 cites W2178996695 @default.
• W2038230871 cites W2343480460 @default.
• W2038230871 cites W2429365795 @default.
• W2038230871 cites W4211239492 @default.
• W2038230871 doi "https://doi.org/10.1006/viro.2000.0219" @default.
• W2038230871 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7131280" @default.
• W2038230871 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10725213" @default.
• W2038230871 hasPublicationYear "2000" @default.
• W2038230871 type Work @default.
• W2038230871 sameAs 2038230871 @default.
• W2038230871 citedByCount "60" @default.
• W2038230871 countsByYear W20382308712012 @default.
• W2038230871 countsByYear W20382308712013 @default.
• W2038230871 countsByYear W20382308712014 @default.
• W2038230871 countsByYear W20382308712015 @default.
• W2038230871 countsByYear W20382308712018 @default.
• W2038230871 countsByYear W20382308712019 @default.
• W2038230871 countsByYear W20382308712020 @default.
• W2038230871 countsByYear W20382308712021 @default.

• next