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- W2038239110 abstract "Previous studies have demonstrated that chondrocyte differentiation can be stimulated by cyclic mechanical compression of chick limb bud cell-agarose constructs. This study reveals that medium collected from these compressed cultures promotes chondrocyte differentiation of non-compressed cells to approximately the same extent as compression alone. In micromass cultures of chick limb bud cells, incubation with medium from compressed cells significantly enhanced cell proliferation and the average rate of proteoglycan synthesis in a dose-dependent manner. These findings indicate that the response of undifferentiated chick limb bud cells to mechanical loading involves the secretion of one or more soluble differentiation factors. The chondrogenic activity of the conditioned medium was substantially inhibited by passage through a filter with a nominal molecular-weight cutoff (MWCO) of 10 kDa but not inhibited when using a MWCO of 30 kDa, suggesting that at least one of these factors has a molecular mass between 10 and 30 kDa." @default.
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- W2038239110 date "2002-09-01" @default.
- W2038239110 modified "2023-09-25" @default.
- W2038239110 title "Conditioned medium of mechanically compressed chick limb bud cells promotes chondrocyte differentiation" @default.
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- W2038239110 doi "https://doi.org/10.1007/s007760200096" @default.
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