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• W2038246105 abstract "A microscale method for purines involved in intracellular signaling and energy metabolism, including ADP, ATP, cyclic-AMP, NADH and GTP, was developed. The analytes were separated on a fused-silica capillary liquid chromatography column (50 microm inner diameter by 25 cm long) packed with 7 microm reversed-phase particles and detected with a carbon fiber cylinder microelectrode at +1.50 V versus Ag/AgCl reference electrode. With an acetonitrile gradient, the separation was carried out within 15 min. With a 100 nl injection volume, the detection limits varied from 0.9 to 8 fmol depending upon the analyte. The low detection limits make the method suitable for analysis of small tissue samples. As a demonstration of the method, islets of Langerhans were analyzed for their adenosine-related messenger content." @default.
• W2038246105 created "2016-06-24" @default.
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• W2038246105 date "2003-01-01" @default.
• W2038246105 modified "2023-09-23" @default.
• W2038246105 title "Microscale determination of purines in tissue samples by capillary liquid chromatography with electrochemical detection" @default.
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• W2038246105 doi "https://doi.org/10.1039/b303949h" @default.
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