FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2038260573> ?p ?o ?g. }

• W2038260573 endingPage "124" @default.
• W2038260573 startingPage "119" @default.
• W2038260573 abstract "Our goal is to develop a general transduction system for G-protein coupled receptors (GPCRs). GPCRs are present in most eukaryote cells and transduce diverse extracellular signals. GPCRs comprise not only the largest class of integral membrane receptors but also the largest class of targets for therapeutic drugs. In all cases studied, binding of ligand to a GPCR leads to a sub-nanometer intramolecular rearrangement. Here, we report the creation of a novel chimaeric BRET-based biosensor by insertion of sequences encoding a bioluminescent donor and a fluorescent acceptor protein into the primary sequence of a GPCR. The BRET2-ODR-10 biosensor was expressed in membranes of Saccharomyces cerevisiae. Assays conducted on isolated membranes indicated an EC50 in the femtomolar range for diacetyl. The response was ligand-specific and was abolished by a single point mutation in the receptor sequence. Novel BRET-GPCR biosensors of this type have potential application in many fields including explosive detection, quality control of food and beverage production, clinical diagnosis and drug discovery." @default.
• W2038260573 created "2016-06-24" @default.
• W2038260573 creator A5012537927 @default.
• W2038260573 creator A5032764121 @default.
• W2038260573 creator A5071179921 @default.
• W2038260573 creator A5080553037 @default.
• W2038260573 creator A5083334580 @default.
• W2038260573 creator A5083780164 @default.
• W2038260573 date "2011-11-01" @default.
• W2038260573 modified "2023-10-07" @default.
• W2038260573 title "Greatly enhanced detection of a volatile ligand at femtomolar levels using bioluminescence resonance energy transfer (BRET)" @default.
• W2038260573 cites W1511344849 @default.
• W2038260573 cites W1592607632 @default.
• W2038260573 cites W1805429580 @default.
• W2038260573 cites W1968001320 @default.
• W2038260573 cites W1974162074 @default.
• W2038260573 cites W1975721509 @default.
• W2038260573 cites W1976548894 @default.
• W2038260573 cites W1981163152 @default.
• W2038260573 cites W1984127042 @default.
• W2038260573 cites W1986080997 @default.
• W2038260573 cites W1989814102 @default.
• W2038260573 cites W1992404679 @default.
• W2038260573 cites W1995112717 @default.
• W2038260573 cites W2009414324 @default.
• W2038260573 cites W2022221238 @default.
• W2038260573 cites W2022948757 @default.
• W2038260573 cites W2027222713 @default.
• W2038260573 cites W2032988762 @default.
• W2038260573 cites W2037427470 @default.
• W2038260573 cites W2044846525 @default.
• W2038260573 cites W2064416689 @default.
• W2038260573 cites W2065468208 @default.
• W2038260573 cites W2080569504 @default.
• W2038260573 cites W2082760954 @default.
• W2038260573 cites W2088667287 @default.
• W2038260573 cites W2096028625 @default.
• W2038260573 cites W2097186487 @default.
• W2038260573 cites W2127068865 @default.
• W2038260573 cites W2136672278 @default.
• W2038260573 cites W2148211715 @default.
• W2038260573 cites W4236323944 @default.
• W2038260573 doi "https://doi.org/10.1016/j.bios.2011.08.004" @default.
• W2038260573 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21873043" @default.
• W2038260573 hasPublicationYear "2011" @default.
• W2038260573 type Work @default.
• W2038260573 sameAs 2038260573 @default.
• W2038260573 citedByCount "54" @default.
• W2038260573 countsByYear W20382605732012 @default.
• W2038260573 countsByYear W20382605732013 @default.
• W2038260573 countsByYear W20382605732014 @default.
• W2038260573 countsByYear W20382605732015 @default.
• W2038260573 countsByYear W20382605732016 @default.
• W2038260573 countsByYear W20382605732017 @default.
• W2038260573 countsByYear W20382605732018 @default.
• W2038260573 countsByYear W20382605732019 @default.
• W2038260573 countsByYear W20382605732020 @default.
• W2038260573 countsByYear W20382605732021 @default.
• W2038260573 countsByYear W20382605732022 @default.
• W2038260573 crossrefType "journal-article" @default.
• W2038260573 hasAuthorship W2038260573A5012537927 @default.
• W2038260573 hasAuthorship W2038260573A5032764121 @default.
• W2038260573 hasAuthorship W2038260573A5071179921 @default.
• W2038260573 hasAuthorship W2038260573A5080553037 @default.
• W2038260573 hasAuthorship W2038260573A5083334580 @default.
• W2038260573 hasAuthorship W2038260573A5083780164 @default.
• W2038260573 hasBestOaLocation W20382605731 @default.
• W2038260573 hasConcept C116569031 @default.
• W2038260573 hasConcept C121332964 @default.
• W2038260573 hasConcept C12554922 @default.
• W2038260573 hasConcept C135285700 @default.
• W2038260573 hasConcept C160756335 @default.
• W2038260573 hasConcept C170493617 @default.
• W2038260573 hasConcept C185592680 @default.
• W2038260573 hasConcept C41625074 @default.
• W2038260573 hasConcept C5176359 @default.
• W2038260573 hasConcept C55493867 @default.
• W2038260573 hasConcept C62520636 @default.
• W2038260573 hasConcept C70721500 @default.
• W2038260573 hasConcept C86803240 @default.
• W2038260573 hasConcept C91881484 @default.
• W2038260573 hasConcept C96305047 @default.
• W2038260573 hasConceptScore W2038260573C116569031 @default.
• W2038260573 hasConceptScore W2038260573C121332964 @default.
• W2038260573 hasConceptScore W2038260573C12554922 @default.
• W2038260573 hasConceptScore W2038260573C135285700 @default.
• W2038260573 hasConceptScore W2038260573C160756335 @default.
• W2038260573 hasConceptScore W2038260573C170493617 @default.
• W2038260573 hasConceptScore W2038260573C185592680 @default.
• W2038260573 hasConceptScore W2038260573C41625074 @default.
• W2038260573 hasConceptScore W2038260573C5176359 @default.
• W2038260573 hasConceptScore W2038260573C55493867 @default.
• W2038260573 hasConceptScore W2038260573C62520636 @default.
• W2038260573 hasConceptScore W2038260573C70721500 @default.
• W2038260573 hasConceptScore W2038260573C86803240 @default.
• W2038260573 hasConceptScore W2038260573C91881484 @default.
• W2038260573 hasConceptScore W2038260573C96305047 @default.
• W2038260573 hasIssue "1" @default.

• next