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• W2038261978 abstract "With the rapid-freeze, deep-etch replica technique, the structural conformations of outer dynein arms in demembranated cilia from Tetrahymena were analyzed under two different conditions, i.e., in the absence of ATP and in the presence of ATP and vanadate. In the absence of ATP, the lateral view of axonemes was characterized by the egg-shaped outer dynein arms, which showed a slightly baseward tilt with a mean inclination of 11.1 degrees +/- 3.4 degrees SD from the perpendicular to the doublet microtubules. On the other hand, in the presence of 1 mM ATP and 100 microM vanadate, the outer arms were extended and slender and showed an increased baseward tilt with a mean inclination of 31.6 degrees +/- 4.9 degrees SD. In ATP-activated axonemes, these two types of arms coexisted, each type occurring in groups along one row of outer arms. These findings strongly suggest that the interdoublet sliding is caused by dynamic structural changes of dynein arms that follow the hydrolysis of ATP." @default.
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• W2038261978 date "1983-05-01" @default.
• W2038261978 modified "2023-09-28" @default.
• W2038261978 title "ATP-dependent structural changes of the outer dynein arm in Tetrahymena cilia: a freeze-etch replica study." @default.
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• W2038261978 doi "https://doi.org/10.1083/jcb.96.5.1480" @default.
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