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- W2038327855 abstract "Based on a new reaction—complex formation, with spectral shift, between phosphomolybdate and the dye, Methyl Green 00—an automatic method has been developed for the determination of inorganic phosphate. The advantages of the method are its very high sensitivity when compared with the existing methods, its extreme simplicity and the very short reaction time (2.5 min) under relatively mild conditions (1.25 N HCl). The hydrolysis of labile phosphates was found negligible. The reproducibility of the method is excellent (relative S.D. = ±0.91%). The method has been applied to the automatic determination of inorganic phosphate in serum. The higher sensitivity results in the uptake of much less sample and the simplicity makes serum phosphate determination less troublesome and less time consuming. In ten consecutive analyses of a serum sample containing 5.59 mg % PO4P the standard deviation was found to be ±0.054. The analysis of 6 different control sera showed an excellent accuracy. Phosphate added to these sera could be completely recovered (99.8–101.5%)." @default.
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- W2038327855 title "New and sensitive reaction for automatic determination of inorganic phosphate and its application to serum" @default.
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- W2038327855 doi "https://doi.org/10.1016/0003-2697(70)90447-1" @default.
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