FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2038384001> ?p ?o ?g. }

• W2038384001 endingPage "e14239" @default.
• W2038384001 startingPage "e14239" @default.
• W2038384001 abstract "Trypanosomes can synthesize polyunsaturated fatty acids. Previously, we have shown that they possess stearoyl-CoA desaturase (SCD) and oleate desaturase (OD) to convert stearate (C18) into oleate (C18:1) and linoleate (C18:2), respectively. Here we examine if OD is essential to these parasites.Cultured procyclic (insect-stage) form (PCF) and bloodstream-form (BSF) Trypanosoma brucei cells were treated with 12- and 13-thiastearic acid (12-TS and 13-TS), inhibitors of OD, and the expression of the enzyme was knocked down by RNA interference. The phenotype of these cells was studied.Growth of PCF T. brucei was totally inhibited by 100 µM of 12-TS and 13-TS, with EC(50) values of 40±2 and 30±2 µM, respectively. The BSF was more sensitive, with EC(50) values of 7±3 and 2±1 µM, respectively. This growth phenotype was due to the inhibitory effect of thiastearates on OD and, to a lesser extent, on SCD. The enzyme inhibition caused a drop in total unsaturated fatty-acid level of the cells, with a slight increase in oleate but a drastic decrease in linoleate level, most probably affecting membrane fluidity. After knocking down OD expression in PCF, the linoleate content was notably reduced, whereas that of oleate drastically increased, maintaining the total unsaturated fatty-acid level unchanged. Interestingly, the growth phenotype of the RNAi-induced cells was similar to that found for thiastearate-treated trypanosomes, with the former cells growing twofold slower than the latter ones, indicating that the linoleate content itself and not only fluidity could be essential for normal membrane functionality. A similar deleterious effect was found after RNAi in BSF, even with a mere 8% reduction of OD activity, indicating that its full activity is essential.As OD is essential for trypanosomes and is not present in mammalian cells, it is a promising target for chemotherapy of African trypanosomiasis." @default.
• W2038384001 created "2016-06-24" @default.
• W2038384001 creator A5010053252 @default.
• W2038384001 creator A5010885639 @default.
• W2038384001 creator A5028495503 @default.
• W2038384001 creator A5040485184 @default.
• W2038384001 creator A5047521794 @default.
• W2038384001 creator A5059608367 @default.
• W2038384001 creator A5069140798 @default.
• W2038384001 creator A5080380194 @default.
• W2038384001 creator A5080829661 @default.
• W2038384001 creator A5082751895 @default.
• W2038384001 date "2010-12-06" @default.
• W2038384001 modified "2023-09-23" @default.
• W2038384001 title "Genetic and Chemical Evaluation of Trypanosoma brucei Oleate Desaturase as a Candidate Drug Target" @default.
• W2038384001 cites W1751804109 @default.
• W2038384001 cites W1934961590 @default.
• W2038384001 cites W1968069269 @default.
• W2038384001 cites W1969608786 @default.
• W2038384001 cites W1971437832 @default.
• W2038384001 cites W1971807222 @default.
• W2038384001 cites W1973182554 @default.
• W2038384001 cites W1973767731 @default.
• W2038384001 cites W1979824753 @default.
• W2038384001 cites W1985799174 @default.
• W2038384001 cites W1988861379 @default.
• W2038384001 cites W2007167713 @default.
• W2038384001 cites W2011441703 @default.
• W2038384001 cites W2023015147 @default.
• W2038384001 cites W2031801843 @default.
• W2038384001 cites W2033184489 @default.
• W2038384001 cites W2053640763 @default.
• W2038384001 cites W2058608211 @default.
• W2038384001 cites W2065464302 @default.
• W2038384001 cites W2098237000 @default.
• W2038384001 cites W2116350092 @default.
• W2038384001 cites W2152311269 @default.
• W2038384001 cites W2161988080 @default.
• W2038384001 doi "https://doi.org/10.1371/journal.pone.0014239" @default.
• W2038384001 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2997783" @default.
• W2038384001 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21151902" @default.
• W2038384001 hasPublicationYear "2010" @default.
• W2038384001 type Work @default.
• W2038384001 sameAs 2038384001 @default.
• W2038384001 citedByCount "12" @default.
• W2038384001 countsByYear W20383840012012 @default.
• W2038384001 countsByYear W20383840012013 @default.
• W2038384001 countsByYear W20383840012014 @default.
• W2038384001 countsByYear W20383840012015 @default.
• W2038384001 countsByYear W20383840012017 @default.
• W2038384001 countsByYear W20383840012018 @default.
• W2038384001 countsByYear W20383840012021 @default.
• W2038384001 crossrefType "journal-article" @default.
• W2038384001 hasAuthorship W2038384001A5010053252 @default.
• W2038384001 hasAuthorship W2038384001A5010885639 @default.
• W2038384001 hasAuthorship W2038384001A5028495503 @default.
• W2038384001 hasAuthorship W2038384001A5040485184 @default.
• W2038384001 hasAuthorship W2038384001A5047521794 @default.
• W2038384001 hasAuthorship W2038384001A5059608367 @default.
• W2038384001 hasAuthorship W2038384001A5069140798 @default.
• W2038384001 hasAuthorship W2038384001A5080380194 @default.
• W2038384001 hasAuthorship W2038384001A5080829661 @default.
• W2038384001 hasAuthorship W2038384001A5082751895 @default.
• W2038384001 hasBestOaLocation W20383840011 @default.
• W2038384001 hasConcept C104317684 @default.
• W2038384001 hasConcept C127716648 @default.
• W2038384001 hasConcept C150194340 @default.
• W2038384001 hasConcept C166703698 @default.
• W2038384001 hasConcept C181199279 @default.
• W2038384001 hasConcept C19038510 @default.
• W2038384001 hasConcept C2777129432 @default.
• W2038384001 hasConcept C2779502636 @default.
• W2038384001 hasConcept C2908932551 @default.
• W2038384001 hasConcept C543025807 @default.
• W2038384001 hasConcept C55493867 @default.
• W2038384001 hasConcept C67705224 @default.
• W2038384001 hasConcept C86803240 @default.
• W2038384001 hasConceptScore W2038384001C104317684 @default.
• W2038384001 hasConceptScore W2038384001C127716648 @default.
• W2038384001 hasConceptScore W2038384001C150194340 @default.
• W2038384001 hasConceptScore W2038384001C166703698 @default.
• W2038384001 hasConceptScore W2038384001C181199279 @default.
• W2038384001 hasConceptScore W2038384001C19038510 @default.
• W2038384001 hasConceptScore W2038384001C2777129432 @default.
• W2038384001 hasConceptScore W2038384001C2779502636 @default.
• W2038384001 hasConceptScore W2038384001C2908932551 @default.
• W2038384001 hasConceptScore W2038384001C543025807 @default.
• W2038384001 hasConceptScore W2038384001C55493867 @default.
• W2038384001 hasConceptScore W2038384001C67705224 @default.
• W2038384001 hasConceptScore W2038384001C86803240 @default.
• W2038384001 hasIssue "12" @default.
• W2038384001 hasLocation W20383840011 @default.
• W2038384001 hasLocation W20383840012 @default.
• W2038384001 hasLocation W20383840013 @default.
• W2038384001 hasLocation W20383840014 @default.
• W2038384001 hasLocation W20383840015 @default.
• W2038384001 hasOpenAccess W2038384001 @default.
• W2038384001 hasPrimaryLocation W20383840011 @default.

• next