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- W2038436610 abstract "The distribution of ecdysteroid binding sites in the hypodermal tissue of crayfish (Procambarus clarkii) was examined autoradiographically in correlation with the molting stage. The radiolabeled hormone analogue ponasterone A (25-deoxy-20-hydroxyecdysone) and thaw-mount autoradiographic techniques were used. These techniques enabled us to prevent the dislocation of soluble ponasterone A. Ecdysteroid binding sites were demonstrated only at certain molting stages, the small gastrolith period (Proecdysis D0 to D2 early). Ponasterone A binding sites appeared in nuclei and cytoplasm of epidermis, Leydig cells and pillar like cells through the stages. The notable appearance of nuclear ecdysteroid binding sites at proecdysis stage D0 coincided with the induction of calcium reabsorption from the exoskeleton, suggesting a strong correlation of the two events. The findings also suggest that the cells involved in calcium transport are targets for ecdysteroids." @default.
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- W2038436610 date "1992-01-01" @default.
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- W2038436610 title "Ponasterone A binding sites in hypodermis during the molting cycle of crayfish Procambarus clarkii." @default.
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- W2038436610 doi "https://doi.org/10.1267/ahc.25.505" @default.
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