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- W2038437739 abstract "Immunoassay for an anti-human IgG antibody and a human IgG is presented. Thin-layer potentiometry, i.e., a combination of fluoride ion selective electrode and silver chloride coated silver-plate reference electrode was used to monitor fluoride release from the multilamellar liposome. Human IgG was attached to the surface of liposome membrane by the use of cross-linking reagent N-hydroxysuccinimidyl3-(2-pyridyldithio)propionate (SPDP). The antigen/antibody/complement reaction triggered the formation of channel-like holes across the liposome membrane which enabled entrapped fluoride anion flow through the hole. The fluoride ion release was specific for anti-human IgG and depended on the presence of complement. By the use of human IgG pendant liposomes, determination of about 10-14mol of anti-human IgG (rabbit) and 10-14mol human IgG was feasible." @default.
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- W2038437739 date "1986-12-01" @default.
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- W2038437739 title "Immunoassay Using Ion Selective Electrode and Protein Pendant Liposomes" @default.
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- W2038437739 doi "https://doi.org/10.2116/analsci.2.523" @default.
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