FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2038470175> ?p ?o ?g. }

• W2038470175 endingPage "3744" @default.
• W2038470175 startingPage "3735" @default.
• W2038470175 abstract "All retroviruses package two copies of their genomes during virus assembly, both of which are required for strand transfer-mediated recombination during reverse transcription. Genome packaging is mediated by interactions between the nucleocapsid (NC) domains of assembling Gag polyproteins and an RNA packaging signal, located near the 5' end of the genome, called Psi. We recently discovered that the NC protein of the Moloney murine leukemia virus (MLV) can bind with high affinity to conserved UCUG elements within the MLV packaging signal [D'Souza, V., and Summers, M. F. (2004) Nature 431, 586-590]. Selective binding to dimeric RNA is regulated by a conformational RNA switch, in which the UCUG elements are sequestered by base pairing in the monomeric RNA and do not bind NC, but become exposed for NC binding upon dimerization. Dimerization-dependent structural changes occur in other regions of the Psi-site, exposing guanosine-containing segments that might also bind NC. Here we demonstrate that short RNAs containing three such sequences, ACAG, UUUG, and UCCG, can bind NC with significant affinity (K(d) = 94-315 nM). Titration experiments with oligoribonucleotides of varying lengths and compositions, combined with NMR-based structural studies, reveal that binding is strictly dependent on the presence of an unpaired guanosine, and that relative binding affinities can vary by more than 1 order of magnitude depending on the nature of the three upstream nucleotides. Binding is enhanced in short RNAs containing terminal phosphates, indicating that electrostatic interactions contribute significantly to binding. Our findings extend a previously published model for genome recognition, in which the NC domains of assembling Gag molecules interact with multiple X(i-3)-X(i-2)-X(i-1)-G(i) elements (X is a variable nucleotide) that appear to be preferentially exposed in the dimeric RNA." @default.
• W2038470175 created "2016-06-24" @default.
• W2038470175 creator A5002027818 @default.
• W2038470175 creator A5025778528 @default.
• W2038470175 creator A5036928216 @default.
• W2038470175 creator A5042731451 @default.
• W2038470175 date "2005-02-18" @default.
• W2038470175 modified "2023-09-27" @default.
• W2038470175 title "Composition and Sequence-Dependent Binding of RNA to the Nucleocapsid Protein of Moloney Murine Leukemia Virus^," @default.
• W2038470175 cites W1492183414 @default.
• W2038470175 cites W1512553008 @default.
• W2038470175 cites W1520951540 @default.
• W2038470175 cites W1584076968 @default.
• W2038470175 cites W1605873417 @default.
• W2038470175 cites W1661066019 @default.
• W2038470175 cites W1806634181 @default.
• W2038470175 cites W1822619347 @default.
• W2038470175 cites W1875262379 @default.
• W2038470175 cites W1939899968 @default.
• W2038470175 cites W1978743038 @default.
• W2038470175 cites W1997624484 @default.
• W2038470175 cites W2004222431 @default.
• W2038470175 cites W2019227662 @default.
• W2038470175 cites W2020322347 @default.
• W2038470175 cites W2020357919 @default.
• W2038470175 cites W2048547641 @default.
• W2038470175 cites W2048927241 @default.
• W2038470175 cites W2052933818 @default.
• W2038470175 cites W2074770586 @default.
• W2038470175 cites W2082176639 @default.
• W2038470175 cites W2096352252 @default.
• W2038470175 cites W2096985241 @default.
• W2038470175 cites W2106183011 @default.
• W2038470175 cites W2163604069 @default.
• W2038470175 cites W2169821755 @default.
• W2038470175 cites W2949250147 @default.
• W2038470175 cites W4247566800 @default.
• W2038470175 doi "https://doi.org/10.1021/bi047639q" @default.
• W2038470175 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15751950" @default.
• W2038470175 hasPublicationYear "2005" @default.
• W2038470175 type Work @default.
• W2038470175 sameAs 2038470175 @default.
• W2038470175 citedByCount "43" @default.
• W2038470175 countsByYear W20384701752012 @default.
• W2038470175 countsByYear W20384701752013 @default.
• W2038470175 countsByYear W20384701752014 @default.
• W2038470175 countsByYear W20384701752015 @default.
• W2038470175 countsByYear W20384701752018 @default.
• W2038470175 countsByYear W20384701752019 @default.
• W2038470175 countsByYear W20384701752020 @default.
• W2038470175 countsByYear W20384701752021 @default.
• W2038470175 countsByYear W20384701752022 @default.
• W2038470175 crossrefType "journal-article" @default.
• W2038470175 hasAuthorship W2038470175A5002027818 @default.
• W2038470175 hasAuthorship W2038470175A5025778528 @default.
• W2038470175 hasAuthorship W2038470175A5036928216 @default.
• W2038470175 hasAuthorship W2038470175A5042731451 @default.
• W2038470175 hasConcept C104317684 @default.
• W2038470175 hasConcept C107824862 @default.
• W2038470175 hasConcept C153911025 @default.
• W2038470175 hasConcept C185592680 @default.
• W2038470175 hasConcept C2522874641 @default.
• W2038470175 hasConcept C2777995097 @default.
• W2038470175 hasConcept C2779562191 @default.
• W2038470175 hasConcept C512185932 @default.
• W2038470175 hasConcept C54355233 @default.
• W2038470175 hasConcept C552990157 @default.
• W2038470175 hasConcept C55493867 @default.
• W2038470175 hasConcept C67705224 @default.
• W2038470175 hasConcept C86803240 @default.
• W2038470175 hasConceptScore W2038470175C104317684 @default.
• W2038470175 hasConceptScore W2038470175C107824862 @default.
• W2038470175 hasConceptScore W2038470175C153911025 @default.
• W2038470175 hasConceptScore W2038470175C185592680 @default.
• W2038470175 hasConceptScore W2038470175C2522874641 @default.
• W2038470175 hasConceptScore W2038470175C2777995097 @default.
• W2038470175 hasConceptScore W2038470175C2779562191 @default.
• W2038470175 hasConceptScore W2038470175C512185932 @default.
• W2038470175 hasConceptScore W2038470175C54355233 @default.
• W2038470175 hasConceptScore W2038470175C552990157 @default.
• W2038470175 hasConceptScore W2038470175C55493867 @default.
• W2038470175 hasConceptScore W2038470175C67705224 @default.
• W2038470175 hasConceptScore W2038470175C86803240 @default.
• W2038470175 hasIssue "10" @default.
• W2038470175 hasLocation W20384701751 @default.
• W2038470175 hasLocation W20384701752 @default.
• W2038470175 hasOpenAccess W2038470175 @default.
• W2038470175 hasPrimaryLocation W20384701751 @default.
• W2038470175 hasRelatedWork W1485780383 @default.
• W2038470175 hasRelatedWork W1596097154 @default.
• W2038470175 hasRelatedWork W1634126162 @default.
• W2038470175 hasRelatedWork W1657463452 @default.
• W2038470175 hasRelatedWork W1976736675 @default.
• W2038470175 hasRelatedWork W2002473487 @default.
• W2038470175 hasRelatedWork W2076093312 @default.
• W2038470175 hasRelatedWork W2088503398 @default.
• W2038470175 hasRelatedWork W2120128263 @default.
• W2038470175 hasRelatedWork W1603456707 @default.

• next