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W2038475433 abstract "The effect of tetranitromethane on the cortisol binding activity of human transcortin has been investigated. This reagent induced a decrease of activity concomitant with nitration of tyrosine residues. An oxidation of sulphydryl groups was also observed but had no implication on cortisol binding. The nitration was specifically oriented in the site at pH6 and with low concentrations of reagent; under these conditions, a single essential tyrosine per molecule of transcortin seems implicated in cortisol binding. The absence of denaturation in modified transcortin was checked by circular dichroism spectra and polyacrylamide gel electrophoresis. Site specificity was demonstrated by full protection with cortisol against inactivation." @default.
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W2038475433 date "1982-02-22" @default.
W2038475433 modified "2023-10-18" @default.
W2038475433 title "Modification of human transcortin by tetranitromethaneEvidence for the implication of a tyrosine residue in cortisol binding" @default.
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W2038475433 doi "https://doi.org/10.1016/s0300-9084(82)80417-3" @default.
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