FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2038482100> ?p ?o ?g. }

• W2038482100 endingPage "527" @default.
• W2038482100 startingPage "517" @default.
• W2038482100 abstract "This study describes the development and application of a bioaffinity chromatographic system for the one-step purification of an NADP(+)-dependent secondary alcohol dehydrogenase from the obligate anaerobe, Thermoanaerobacter brockii (TBADH, EC 1.1.1.2). The general approach is based upon improving the selectivity of immobilized cofactor derivatives (general ligand approach to bioaffinity chromatography) through using soluble enzyme-specific substrate analogues in irrigants to promote biospecific adsorption (the kinetic locking-on tactic). Specifically, the following is described: Evaluation of 8'-azo-linked, C(8)-linked, N(1)-linked, and N(6)-linked immobilized NADP(+) derivatives for use with the kinetic locking-on strategy for bioaffinity purification of TBADH; evaluation of 2', 5'-ADP as a stripping ligand for TBADH bioaffinity purifications using an 8'-azo-linked immobilized NADP(+) derivative in the locking-on mode; and application of the developed bioaffinity chromatographic system to the purification of TBADH from a crude cellular extract. Surprizingly, of the four immobilized NADP(+) derivatives investigated, only the 8'-azo-linked immobilized NADP(+) derivative proved effective for TBADH affinity purification when used in conjunction with pyrazole (a competitive inhibitor of TBADH activity) as the locking-on ligand. This is in contrast to other NADP(+)-dependent dehydrogenases where the immobilized N(6)-linked cofactor proved to be suitable. While the one-step purification of TBADH to electrophoretic homogeneity is described in the present study (92% yield), results from the model chromatographic studies point to improvements that could be made to the immobilized cofactor derivative to improve its suitability for TBADH bioaffinity purification and to facilitate future large scale protein purification operations." @default.
• W2038482100 created "2016-06-24" @default.
• W2038482100 creator A5025106796 @default.
• W2038482100 creator A5045893041 @default.
• W2038482100 date "2002-01-09" @default.
• W2038482100 modified "2023-10-18" @default.
• W2038482100 title "Bioaffinity purification of NADP+-dependent dehydrogenases: Studies with alcohol dehydrogenase fromThermoanaerobacter brockii" @default.
• W2038482100 cites W132340928 @default.
• W2038482100 cites W1528054778 @default.
• W2038482100 cites W1749144760 @default.
• W2038482100 cites W1886064066 @default.
• W2038482100 cites W1967531130 @default.
• W2038482100 cites W1968314606 @default.
• W2038482100 cites W1968479752 @default.
• W2038482100 cites W1971432116 @default.
• W2038482100 cites W1972170989 @default.
• W2038482100 cites W1974489131 @default.
• W2038482100 cites W1983792260 @default.
• W2038482100 cites W1986550844 @default.
• W2038482100 cites W1992944463 @default.
• W2038482100 cites W2022081324 @default.
• W2038482100 cites W2022838644 @default.
• W2038482100 cites W2027598442 @default.
• W2038482100 cites W2029720920 @default.
• W2038482100 cites W2037086510 @default.
• W2038482100 cites W2040668425 @default.
• W2038482100 cites W2047552112 @default.
• W2038482100 cites W2048775838 @default.
• W2038482100 cites W2049572208 @default.
• W2038482100 cites W2058579906 @default.
• W2038482100 cites W2058916242 @default.
• W2038482100 cites W2064625880 @default.
• W2038482100 cites W2071016193 @default.
• W2038482100 cites W2093426281 @default.
• W2038482100 cites W2100837269 @default.
• W2038482100 cites W2119449094 @default.
• W2038482100 cites W2125097548 @default.
• W2038482100 cites W2171750678 @default.
• W2038482100 cites W2953165354 @default.
• W2038482100 cites W34923332 @default.
• W2038482100 cites W39136819 @default.
• W2038482100 cites W4293247451 @default.
• W2038482100 doi "https://doi.org/10.1002/bit.10079" @default.
• W2038482100 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11788950" @default.
• W2038482100 hasPublicationYear "2002" @default.
• W2038482100 type Work @default.
• W2038482100 sameAs 2038482100 @default.
• W2038482100 citedByCount "6" @default.
• W2038482100 countsByYear W20384821002015 @default.
• W2038482100 countsByYear W20384821002017 @default.
• W2038482100 crossrefType "journal-article" @default.
• W2038482100 hasAuthorship W2038482100A5025106796 @default.
• W2038482100 hasAuthorship W2038482100A5045893041 @default.
• W2038482100 hasConcept C116569031 @default.
• W2038482100 hasConcept C161790260 @default.
• W2038482100 hasConcept C170493617 @default.
• W2038482100 hasConcept C179247698 @default.
• W2038482100 hasConcept C181199279 @default.
• W2038482100 hasConcept C185592680 @default.
• W2038482100 hasConcept C18903297 @default.
• W2038482100 hasConcept C191503008 @default.
• W2038482100 hasConcept C197957613 @default.
• W2038482100 hasConcept C21951064 @default.
• W2038482100 hasConcept C2776317432 @default.
• W2038482100 hasConcept C2777289219 @default.
• W2038482100 hasConcept C2777344606 @default.
• W2038482100 hasConcept C43617362 @default.
• W2038482100 hasConcept C55493867 @default.
• W2038482100 hasConcept C65024703 @default.
• W2038482100 hasConcept C75520062 @default.
• W2038482100 hasConcept C86803240 @default.
• W2038482100 hasConceptScore W2038482100C116569031 @default.
• W2038482100 hasConceptScore W2038482100C161790260 @default.
• W2038482100 hasConceptScore W2038482100C170493617 @default.
• W2038482100 hasConceptScore W2038482100C179247698 @default.
• W2038482100 hasConceptScore W2038482100C181199279 @default.
• W2038482100 hasConceptScore W2038482100C185592680 @default.
• W2038482100 hasConceptScore W2038482100C18903297 @default.
• W2038482100 hasConceptScore W2038482100C191503008 @default.
• W2038482100 hasConceptScore W2038482100C197957613 @default.
• W2038482100 hasConceptScore W2038482100C21951064 @default.
• W2038482100 hasConceptScore W2038482100C2776317432 @default.
• W2038482100 hasConceptScore W2038482100C2777289219 @default.
• W2038482100 hasConceptScore W2038482100C2777344606 @default.
• W2038482100 hasConceptScore W2038482100C43617362 @default.
• W2038482100 hasConceptScore W2038482100C55493867 @default.
• W2038482100 hasConceptScore W2038482100C65024703 @default.
• W2038482100 hasConceptScore W2038482100C75520062 @default.
• W2038482100 hasConceptScore W2038482100C86803240 @default.
• W2038482100 hasIssue "5" @default.
• W2038482100 hasLocation W20384821001 @default.
• W2038482100 hasLocation W20384821002 @default.
• W2038482100 hasOpenAccess W2038482100 @default.
• W2038482100 hasPrimaryLocation W20384821001 @default.
• W2038482100 hasRelatedWork W2003327451 @default.
• W2038482100 hasRelatedWork W2037702679 @default.
• W2038482100 hasRelatedWork W2049048597 @default.
• W2038482100 hasRelatedWork W2080331064 @default.

• next