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- W2038486593 abstract "Synthesis of fructokinase of Streptomyces violaceoruber has been obtained with a preincubated S-30 fraction from Escherichia coli and RNA from S. violaceoruber. The fructokinase formed was identified by its exceptional catalytic activity at pH 10 and by the similar specificity properties of the native enzyme and the enzyme synthesized in vitro. Its presence was confirmed among the radioactive products of protein synthesis by its transition of molecular weight from 20000 to 80000 in the presence of MgATP and by electrophoresis of purified preparations. The presumptive mRNA of fructokinase contained poly(A) sequences of variable length. Fructokinase mRNA was present in S. violaceoruber when grown with fructose but not with glucose, indicating that the induction of fructokinase is controlled at the level of transcription." @default.
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- W2038486593 date "1981-09-01" @default.
- W2038486593 modified "2023-09-22" @default.
- W2038486593 title "Cell-free Synthesis of Catalytically Active Fructokinase Directed by RNA from Streptomyces violaceoruber Grown with Fructose" @default.
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- W2038486593 doi "https://doi.org/10.1099/00221287-126-1-203" @default.
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