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• W2038530768 abstract "A process of highly stereoselective reduction of ethyl 4-chloro-3-oxobutanoate (COBE) to ethyl (S)-4-chloro-3-hydroxybutanoate ((S)-CHBE) was established, using whole-cells of a fungus, Aureobasidium pullulans CGMCC1244. A high production yield was observed in a biphasic system composed of potassium phosphate buffer (0.1 M, pH 6.5) and dibutylphthalate. The influence of reaction parameters such as shaking speed, volumetric phase ratio, reaction temperature, biomass/substrate ratio and pH was tested. It was found that these parameters considerably influenced the molar conversion and the initial reaction rate, but had no significant effect on the optical purity of the product. Under the optimum conditions, the maximum concentration of (S)-CHBE in the organic layer, the molar conversion and the optical purity reached 56.8 g l−1, 94.5 and 97.7% enantiomeric excess (ee), respectively." @default.
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• W2038530768 date "2006-01-01" @default.
• W2038530768 modified "2023-09-25" @default.
• W2038530768 title "Biocatalytic synthesis of ethyl (S)-4-chloro-3-hydroxy-butanoate in an aqueous-organic solvent biphasic system using Aureobasidium pullulans CGMCC 1244" @default.
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• W2038530768 doi "https://doi.org/10.1016/j.procbio.2005.06.028" @default.
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