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- W2038535696 abstract "Rhipicephalus (Boophilus) microplus is an economically significant pest of livestock in India. Allele-specific polymerase chain reaction (PCR) was employed to detect Phe→Ile substitution in the sodium channel gene in different populations of Rhipicephalus microplus collected from foothills (Kashipur, Pantnagar, Nagla and Dehradun) and high-altitude areas (Pithoragarh, Almora and New Tehri) of Uttarakhand state in northern India. The allele-specific PCR assay revealed 85% larvae to be homozygous susceptible, 3% to be homozygous resistant and 12% to be heterozygous. Homozygous resistant genotypes were recorded in two tick populations (Kashipur, 13% and Pantnagar, 4%); heterozygous genotypes in four tick populations (Kashipur, 45%; Pantnagar, 17%; Almora, 14%; and New Tehri, 3%) and 100% homozygous susceptible genotypic frequencies in three tick populations (Nagla, Dehradun and Pithoragarh). The highest rate of mutant allele (R) occurred in tick populations of Kashipur (35%) (p < 0.05), followed by Pantnagar (13%), Almora (7%) and New Tehri (1%). The results of the study suggested that frequent monitoring of the level of pyrethroid resistance was required to formulate any control strategy against ticks and hence extend the life of the existing acaricide(s)." @default.
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- W2038535696 date "2012-12-01" @default.
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- W2038535696 title "Detecting mutation in sodium channel gene inRhipicephalus(Boophilus)micropluscollected from northern India for genotyping deltamethrin-susceptible and deltamethrin-resistant alleles" @default.
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- W2038535696 doi "https://doi.org/10.1080/01647954.2012.733022" @default.
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