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- W2038543105 abstract "ATP:citrate lyase (ACL) is a major generator of cytosolic acetyl-coenzymeA, which is required for both fatty acid and cholesterol biosynthesis. The human ACL (hACL) cDNA was cloned by RT-PCR, and our results indicate the existence of previously unknown sequence variations in hACL. Expression of the hACL cDNA in Spodoptera frugiperda 9 insect cells resulted in the production of high levels of soluble, active enzyme. The recombinant protein (re-hACL) was purified to homogeneity from the soluble lysate of infected cells and was observed to exist as a tetramer by gel filtration chromatography. Kinetic analyses indicated that the re-hACL and rat ACL have very similar enzymological properties. The facile preparation of milligram quantities of purified, active re-hACL affords the opportunity to characterize the enzyme for structure-based design of hypolipidemic drugs, and to further examine the functional significance of the sequence variations." @default.
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- W2038543105 date "1997-02-01" @default.
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- W2038543105 title "Variant cDNA Sequences of Human ATP:Citrate Lyase: Cloning, Expression, and Purification from Baculovirus-Infected Insect Cells" @default.
- W2038543105 doi "https://doi.org/10.1006/prep.1996.0668" @default.
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