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- W2038543454 abstract "Abstract This study was conducted to determine the effect of Papaver rhoeas L. extract on in vitro maturation (IVM) of sheep oocytes. Sheep ovaries collected from a local abattoir were transported to the laboratory within 2 h after slaughter. The cumulus–oocyte complexes (COCs) were aspirated from follicles (2–6 mm in diameter). Good quality COCs were selected and cultured in TCM 199 supplemented with 0 (control), 25, 50, 100 and 200 μg/ml of P. rhoeas extract. The COCs were incubated at 38.5 °C in humidified atmosphere of 5% CO2 in air for 24 h. After the incubation period, expansion of cumulus cells and the nuclear status of the oocytes were assessed by phase contrast microscopy and Hoechst 33258, respectively. There were no significant differences between treatments in the percentage of cumulus expansion (P > 0.05). The percentage of arrested oocytes at germinal vesicle (GV) stage in the control group was significantly (P" @default.
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- W2038543454 date "2013-08-01" @default.
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- W2038543454 title "Effect of Papaver rhoeas L. extract on in vitro maturation of sheep oocytes" @default.
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- W2038543454 doi "https://doi.org/10.1016/j.smallrumres.2013.06.010" @default.
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