Matches in SemOpenAlex for { <https://semopenalex.org/work/W2038562110> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W2038562110 endingPage "204" @default.
- W2038562110 startingPage "199" @default.
- W2038562110 abstract "In an effort to develop genetically engineered Brucella abortus (BA) vaccines, the genes encoding heat shock proteins (HSPs) GroEL, GroES, and HtrA were cloned and expressed in the BAC-TO-BAC Baculovirus System, and the kinetics of protein expression were analyzed using various insect cell lines in suspension cultures, different cell densities in suspension cultures, multiplicities of infection and recombinant virus replication times. Trichoplusia ni cells expressed only BA HtrA, but Spodoptera frugiperda (Sf9) cells expressed all three recombinant proteins. The best GroEL expression was achieved by infecting 2x10(6) Sf9 cells/ml with an MOI 10 of recombinant virus and harvesting the cells after 96h of virus replication. GroES and HtrA were best expressed when infecting 2x10(6) Sf9 cells/ml with an MOI 1 of recombinant viruses and harvesting the cells after 120h of virus replications. Under these conditions BA recombinant HSPs were expressed as follows: GroEL at 16% of the total cellular proteins (105microg/ml concentration); GroES 2% (15.25microg/ml); and HtrA 8% (84.48microg/ml). This is the first report of cloning and expression of BA genes in the baculovirus system." @default.
- W2038562110 created "2016-06-24" @default.
- W2038562110 creator A5020536750 @default.
- W2038562110 creator A5061032029 @default.
- W2038562110 date "2000-07-01" @default.
- W2038562110 modified "2023-09-25" @default.
- W2038562110 title "Cloning and kinetics of expression of Brucella abortus heat shock proteins by baculovirus recombinants" @default.
- W2038562110 cites W1922446009 @default.
- W2038562110 cites W1975148425 @default.
- W2038562110 cites W2023466305 @default.
- W2038562110 cites W2063450941 @default.
- W2038562110 cites W2087832275 @default.
- W2038562110 cites W2154361055 @default.
- W2038562110 cites W2172466148 @default.
- W2038562110 cites W2175553381 @default.
- W2038562110 cites W2213514015 @default.
- W2038562110 cites W2341022887 @default.
- W2038562110 cites W275572846 @default.
- W2038562110 doi "https://doi.org/10.1016/s0378-1135(00)00203-0" @default.
- W2038562110 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10889410" @default.
- W2038562110 hasPublicationYear "2000" @default.
- W2038562110 type Work @default.
- W2038562110 sameAs 2038562110 @default.
- W2038562110 citedByCount "12" @default.
- W2038562110 countsByYear W20385621102012 @default.
- W2038562110 countsByYear W20385621102015 @default.
- W2038562110 crossrefType "journal-article" @default.
- W2038562110 hasAuthorship W2038562110A5020536750 @default.
- W2038562110 hasAuthorship W2038562110A5061032029 @default.
- W2038562110 hasConcept C103878061 @default.
- W2038562110 hasConcept C104317684 @default.
- W2038562110 hasConcept C121050878 @default.
- W2038562110 hasConcept C153911025 @default.
- W2038562110 hasConcept C159047783 @default.
- W2038562110 hasConcept C173758957 @default.
- W2038562110 hasConcept C199360897 @default.
- W2038562110 hasConcept C205260736 @default.
- W2038562110 hasConcept C2777212814 @default.
- W2038562110 hasConcept C2777773057 @default.
- W2038562110 hasConcept C2778350275 @default.
- W2038562110 hasConcept C2779341487 @default.
- W2038562110 hasConcept C2781140520 @default.
- W2038562110 hasConcept C39060996 @default.
- W2038562110 hasConcept C40767141 @default.
- W2038562110 hasConcept C41008148 @default.
- W2038562110 hasConcept C46522908 @default.
- W2038562110 hasConcept C54355233 @default.
- W2038562110 hasConcept C547475151 @default.
- W2038562110 hasConcept C59822182 @default.
- W2038562110 hasConcept C86803240 @default.
- W2038562110 hasConcept C87190427 @default.
- W2038562110 hasConceptScore W2038562110C103878061 @default.
- W2038562110 hasConceptScore W2038562110C104317684 @default.
- W2038562110 hasConceptScore W2038562110C121050878 @default.
- W2038562110 hasConceptScore W2038562110C153911025 @default.
- W2038562110 hasConceptScore W2038562110C159047783 @default.
- W2038562110 hasConceptScore W2038562110C173758957 @default.
- W2038562110 hasConceptScore W2038562110C199360897 @default.
- W2038562110 hasConceptScore W2038562110C205260736 @default.
- W2038562110 hasConceptScore W2038562110C2777212814 @default.
- W2038562110 hasConceptScore W2038562110C2777773057 @default.
- W2038562110 hasConceptScore W2038562110C2778350275 @default.
- W2038562110 hasConceptScore W2038562110C2779341487 @default.
- W2038562110 hasConceptScore W2038562110C2781140520 @default.
- W2038562110 hasConceptScore W2038562110C39060996 @default.
- W2038562110 hasConceptScore W2038562110C40767141 @default.
- W2038562110 hasConceptScore W2038562110C41008148 @default.
- W2038562110 hasConceptScore W2038562110C46522908 @default.
- W2038562110 hasConceptScore W2038562110C54355233 @default.
- W2038562110 hasConceptScore W2038562110C547475151 @default.
- W2038562110 hasConceptScore W2038562110C59822182 @default.
- W2038562110 hasConceptScore W2038562110C86803240 @default.
- W2038562110 hasConceptScore W2038562110C87190427 @default.
- W2038562110 hasIssue "2" @default.
- W2038562110 hasLocation W20385621101 @default.
- W2038562110 hasLocation W20385621102 @default.
- W2038562110 hasOpenAccess W2038562110 @default.
- W2038562110 hasPrimaryLocation W20385621101 @default.
- W2038562110 hasRelatedWork W2002347070 @default.
- W2038562110 hasRelatedWork W2022850367 @default.
- W2038562110 hasRelatedWork W2032546828 @default.
- W2038562110 hasRelatedWork W2038562110 @default.
- W2038562110 hasRelatedWork W2045215302 @default.
- W2038562110 hasRelatedWork W2051231036 @default.
- W2038562110 hasRelatedWork W2079156087 @default.
- W2038562110 hasRelatedWork W2368564320 @default.
- W2038562110 hasRelatedWork W2760011480 @default.
- W2038562110 hasRelatedWork W2785525020 @default.
- W2038562110 hasVolume "75" @default.
- W2038562110 isParatext "false" @default.
- W2038562110 isRetracted "false" @default.
- W2038562110 magId "2038562110" @default.
- W2038562110 workType "article" @default.