Matches in SemOpenAlex for { <https://semopenalex.org/work/W2038572590> ?p ?o ?g. }
Showing items 1 to 87 of
87
with 100 items per page.
- W2038572590 abstract "abate metastasis dissemination by maintaining lymphatic vessel junctions’ integrity. Methods: The azoxymethane (AOM)/dextran sulfate sodium (DSS) CAC model was used. In addition, colitic mice were orthotopically injected with CT26 colon cancer cells. Modulation of lymphangiogenesis was obtained by systemic inhibition of VEGFR3 or adenoviral overexpression of VEGFC. Tumor density and size were measured at the end of the AOM/DSS protocol by visual, endoscopic and histological inspection. In the xenotransplantation model, liver, lung and draining lymphnodes were processed for metastasis quantification by FACS analysis. Whole mounts of colons were stained to analyse area density and dimension of lymphatic vessels (LVs) within peritumoral and tumoral regions. Moreover, VE-cadherin distribution and expression were studied in vivo on LVs and in vitro on Human intestinal lymphatic endothelial cells (HILEC) stimulated with VEGFC or with a VEGFR3 inhibitor. Results: In both the AOM/DSS and the xenotransplantation models, systemic inhibition of VEGFR3 inhibited lymphangiogenesis in peritumoral and tumoral regions, reducing both area density and LVs dimension. In addition, it reduced tumor density and size, together with an abatement of metastatic dissemination, when compared to untreated animals. In contrast, tumor density and growth, including metastasis formation was enhanced by VEGFC, which in turn increased lymphangiogenesis within the same regions. Whole mount staining showed that VEGFC altered VE-cadherin expression both in vivo and in vitro, whereas VEGFR3 inhibitor kept endothelial junction integrity, thus linking the VEGFC/VEGFR3 pathway to VE-cadherin-dependent metastasis dissemination. Conclusions: Our findings demonstrate that VEGFC/VEGFR3dependent lymphangiogenesis plays a key role both in CAC growth and metastasis dissemination. Our study reveals a novel mechanism of control of lymphatic junction integrity which may be a promising target for the treatment of CAC and the associated metastatic process." @default.
- W2038572590 created "2016-06-24" @default.
- W2038572590 creator A5009081065 @default.
- W2038572590 creator A5035092369 @default.
- W2038572590 creator A5039768297 @default.
- W2038572590 creator A5048959749 @default.
- W2038572590 creator A5065456371 @default.
- W2038572590 creator A5069425218 @default.
- W2038572590 date "2014-02-01" @default.
- W2038572590 modified "2023-09-22" @default.
- W2038572590 title "P017 The effect of intestinal alkaline phosphatase on intestinal epithelial cells, macrophages and chronic colitis in interleukin-10-deficient mice" @default.
- W2038572590 doi "https://doi.org/10.1016/s1873-9946(14)60140-9" @default.
- W2038572590 hasPublicationYear "2014" @default.
- W2038572590 type Work @default.
- W2038572590 sameAs 2038572590 @default.
- W2038572590 citedByCount "0" @default.
- W2038572590 crossrefType "journal-article" @default.
- W2038572590 hasAuthorship W2038572590A5009081065 @default.
- W2038572590 hasAuthorship W2038572590A5035092369 @default.
- W2038572590 hasAuthorship W2038572590A5039768297 @default.
- W2038572590 hasAuthorship W2038572590A5048959749 @default.
- W2038572590 hasAuthorship W2038572590A5065456371 @default.
- W2038572590 hasAuthorship W2038572590A5069425218 @default.
- W2038572590 hasBestOaLocation W20385725901 @default.
- W2038572590 hasConcept C103041312 @default.
- W2038572590 hasConcept C121608353 @default.
- W2038572590 hasConcept C126322002 @default.
- W2038572590 hasConcept C142724271 @default.
- W2038572590 hasConcept C146285616 @default.
- W2038572590 hasConcept C167734588 @default.
- W2038572590 hasConcept C181152851 @default.
- W2038572590 hasConcept C203014093 @default.
- W2038572590 hasConcept C2776636583 @default.
- W2038572590 hasConcept C2777025900 @default.
- W2038572590 hasConcept C2778442404 @default.
- W2038572590 hasConcept C2779013556 @default.
- W2038572590 hasConcept C2780965494 @default.
- W2038572590 hasConcept C2910366181 @default.
- W2038572590 hasConcept C2911091166 @default.
- W2038572590 hasConcept C502942594 @default.
- W2038572590 hasConcept C71924100 @default.
- W2038572590 hasConcept C86803240 @default.
- W2038572590 hasConceptScore W2038572590C103041312 @default.
- W2038572590 hasConceptScore W2038572590C121608353 @default.
- W2038572590 hasConceptScore W2038572590C126322002 @default.
- W2038572590 hasConceptScore W2038572590C142724271 @default.
- W2038572590 hasConceptScore W2038572590C146285616 @default.
- W2038572590 hasConceptScore W2038572590C167734588 @default.
- W2038572590 hasConceptScore W2038572590C181152851 @default.
- W2038572590 hasConceptScore W2038572590C203014093 @default.
- W2038572590 hasConceptScore W2038572590C2776636583 @default.
- W2038572590 hasConceptScore W2038572590C2777025900 @default.
- W2038572590 hasConceptScore W2038572590C2778442404 @default.
- W2038572590 hasConceptScore W2038572590C2779013556 @default.
- W2038572590 hasConceptScore W2038572590C2780965494 @default.
- W2038572590 hasConceptScore W2038572590C2910366181 @default.
- W2038572590 hasConceptScore W2038572590C2911091166 @default.
- W2038572590 hasConceptScore W2038572590C502942594 @default.
- W2038572590 hasConceptScore W2038572590C71924100 @default.
- W2038572590 hasConceptScore W2038572590C86803240 @default.
- W2038572590 hasLocation W20385725901 @default.
- W2038572590 hasOpenAccess W2038572590 @default.
- W2038572590 hasPrimaryLocation W20385725901 @default.
- W2038572590 hasRelatedWork W1489867969 @default.
- W2038572590 hasRelatedWork W1573850291 @default.
- W2038572590 hasRelatedWork W1928341786 @default.
- W2038572590 hasRelatedWork W1971467639 @default.
- W2038572590 hasRelatedWork W1993062169 @default.
- W2038572590 hasRelatedWork W2003321853 @default.
- W2038572590 hasRelatedWork W2039666275 @default.
- W2038572590 hasRelatedWork W2075852744 @default.
- W2038572590 hasRelatedWork W2179901035 @default.
- W2038572590 hasRelatedWork W2228540091 @default.
- W2038572590 hasRelatedWork W2298569278 @default.
- W2038572590 hasRelatedWork W2327045091 @default.
- W2038572590 hasRelatedWork W2364700924 @default.
- W2038572590 hasRelatedWork W2392360781 @default.
- W2038572590 hasRelatedWork W2615584850 @default.
- W2038572590 hasRelatedWork W2948373323 @default.
- W2038572590 hasRelatedWork W2950979380 @default.
- W2038572590 hasRelatedWork W3025093490 @default.
- W2038572590 hasRelatedWork W3034805736 @default.
- W2038572590 hasRelatedWork W3119349791 @default.
- W2038572590 isParatext "false" @default.
- W2038572590 isRetracted "false" @default.
- W2038572590 magId "2038572590" @default.
- W2038572590 workType "article" @default.