FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2038644800> ?p ?o ?g. }

• W2038644800 endingPage "1066" @default.
• W2038644800 startingPage "1060" @default.
• W2038644800 abstract "Due to their high hydrophobicity, it is a challenge to obtain high yields of transmembrane peptides for structural and functional characterization. In the present work, a robust method is developed for the expression, purification and reconstitution of transmembrane peptides, especially for those containing conserved methionines. By using a truncated and mutated glutathione-S-transferase construct as the carrier protein and hydroxylamine (which specifically cleaves the peptide bond between Asn and Gly) as the cleavage reagent, 10 mg of the first transmembrane helix of CorA, a Mg2+ transporter from Mycobacterium tuberculosis, can be conveniently obtained with high purity from 1 L of M9 minimal media under optimized conditions. The biophysical properties of the peptide were studied by circular dichroism and nuclear magnetic resonance spectroscopy, and the results show that this CorA peptide is well folded in detergent micelles and the secondary structure is very similar to that in recent crystal structures. In addition, this CorA construct is oligomeric in perfluoro-octanoic acid micelles. The compatibility with the transmembrane peptides containing conserved methionines, the high yield and the simple process make the present method competitive with other commonly used methods to produce such peptides for structural and functional studies." @default.
• W2038644800 created "2016-06-24" @default.
• W2038644800 creator A5020704363 @default.
• W2038644800 creator A5027395813 @default.
• W2038644800 creator A5030761558 @default.
• W2038644800 creator A5075658290 @default.
• W2038644800 creator A5082080586 @default.
• W2038644800 date "2008-04-01" @default.
• W2038644800 modified "2023-10-16" @default.
• W2038644800 title "Chemical cleavage of fusion proteins for high-level production of transmembrane peptides and protein domains containing conserved methionines" @default.
• W2038644800 cites W1030517862 @default.
• W2038644800 cites W1484927693 @default.
• W2038644800 cites W1597097623 @default.
• W2038644800 cites W1823351566 @default.
• W2038644800 cites W1857017278 @default.
• W2038644800 cites W1969384291 @default.
• W2038644800 cites W1971852550 @default.
• W2038644800 cites W1972065621 @default.
• W2038644800 cites W1978901030 @default.
• W2038644800 cites W1980823735 @default.
• W2038644800 cites W1983761574 @default.
• W2038644800 cites W1985935502 @default.
• W2038644800 cites W2006634380 @default.
• W2038644800 cites W2008196521 @default.
• W2038644800 cites W2014283693 @default.
• W2038644800 cites W2033720224 @default.
• W2038644800 cites W2034672438 @default.
• W2038644800 cites W2034748211 @default.
• W2038644800 cites W2038096428 @default.
• W2038644800 cites W2041999765 @default.
• W2038644800 cites W2043841973 @default.
• W2038644800 cites W2052553094 @default.
• W2038644800 cites W2057112136 @default.
• W2038644800 cites W2068770352 @default.
• W2038644800 cites W2069734146 @default.
• W2038644800 cites W2094531949 @default.
• W2038644800 cites W2104017463 @default.
• W2038644800 cites W2105924921 @default.
• W2038644800 cites W2108981195 @default.
• W2038644800 cites W2114431583 @default.
• W2038644800 cites W2120050247 @default.
• W2038644800 cites W2122056204 @default.
• W2038644800 cites W2131817589 @default.
• W2038644800 cites W2148521301 @default.
• W2038644800 cites W2154801081 @default.
• W2038644800 cites W2160259388 @default.
• W2038644800 cites W2170823043 @default.
• W2038644800 doi "https://doi.org/10.1016/j.bbamem.2007.12.024" @default.
• W2038644800 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18230329" @default.
• W2038644800 hasPublicationYear "2008" @default.
• W2038644800 type Work @default.
• W2038644800 sameAs 2038644800 @default.
• W2038644800 citedByCount "13" @default.
• W2038644800 countsByYear W20386448002012 @default.
• W2038644800 countsByYear W20386448002013 @default.
• W2038644800 countsByYear W20386448002014 @default.
• W2038644800 countsByYear W20386448002018 @default.
• W2038644800 countsByYear W20386448002019 @default.
• W2038644800 countsByYear W20386448002020 @default.
• W2038644800 countsByYear W20386448002021 @default.
• W2038644800 countsByYear W20386448002022 @default.
• W2038644800 crossrefType "journal-article" @default.
• W2038644800 hasAuthorship W2038644800A5020704363 @default.
• W2038644800 hasAuthorship W2038644800A5027395813 @default.
• W2038644800 hasAuthorship W2038644800A5030761558 @default.
• W2038644800 hasAuthorship W2038644800A5075658290 @default.
• W2038644800 hasAuthorship W2038644800A5082080586 @default.
• W2038644800 hasBestOaLocation W20386448001 @default.
• W2038644800 hasConcept C104317684 @default.
• W2038644800 hasConcept C11268172 @default.
• W2038644800 hasConcept C118892022 @default.
• W2038644800 hasConcept C133571119 @default.
• W2038644800 hasConcept C147789679 @default.
• W2038644800 hasConcept C151730666 @default.
• W2038644800 hasConcept C167625842 @default.
• W2038644800 hasConcept C170493617 @default.
• W2038644800 hasConcept C175156509 @default.
• W2038644800 hasConcept C184651966 @default.
• W2038644800 hasConcept C185592680 @default.
• W2038644800 hasConcept C24530287 @default.
• W2038644800 hasConcept C2779281246 @default.
• W2038644800 hasConcept C43369102 @default.
• W2038644800 hasConcept C515207424 @default.
• W2038644800 hasConcept C55493867 @default.
• W2038644800 hasConcept C55988834 @default.
• W2038644800 hasConcept C86803240 @default.
• W2038644800 hasConceptScore W2038644800C104317684 @default.
• W2038644800 hasConceptScore W2038644800C11268172 @default.
• W2038644800 hasConceptScore W2038644800C118892022 @default.
• W2038644800 hasConceptScore W2038644800C133571119 @default.
• W2038644800 hasConceptScore W2038644800C147789679 @default.
• W2038644800 hasConceptScore W2038644800C151730666 @default.
• W2038644800 hasConceptScore W2038644800C167625842 @default.
• W2038644800 hasConceptScore W2038644800C170493617 @default.
• W2038644800 hasConceptScore W2038644800C175156509 @default.
• W2038644800 hasConceptScore W2038644800C184651966 @default.
• W2038644800 hasConceptScore W2038644800C185592680 @default.
• W2038644800 hasConceptScore W2038644800C24530287 @default.

• next