Matches in SemOpenAlex for { <https://semopenalex.org/work/W2038697935> ?p ?o ?g. }
- W2038697935 endingPage "393" @default.
- W2038697935 startingPage "385" @default.
- W2038697935 abstract "We have investigated the topology of the alpha and delta subunits of the nicotinic acetylcholine receptor (AChR) from mammalian muscle synthesized in an in vitro translation system supplemented with dog pancreatic microsomes. Fusion proteins were expressed in which a carboxy-terminal fragment of bovine prolactin was attached downstream of each of the major putative transmembrane domains, M1-M4 and MA, in the AChR subunits. The orientation of the prolactin domain relative to the microsomal membrane was then determined for each protein by a proteolysis protection assay. Since the prolactin domain contains no information which either directs or prevents its translocation, its transmembrane orientation depends solely on sequences within the AChR subunit portion of the fusion protein. When subunit-prolactin fusion proteins with the prolactin domain fused after either M2 or M4 were tested, prolactin-immunoreactive peptides that were larger than the prolactin domain itself were recovered. No prolactin-immunoreactive peptides were recovered after proteolysis of fusion proteins containing prolactin fused after M1, M3, or MA. These results support a model of AChR subunit topology in which M1-M4, but not MA, are transmembrane domains and the carboxy terminus is extracellular." @default.
- W2038697935 created "2016-06-24" @default.
- W2038697935 creator A5028036842 @default.
- W2038697935 creator A5069247857 @default.
- W2038697935 date "1992-01-15" @default.
- W2038697935 modified "2023-10-16" @default.
- W2038697935 title "Expression of fusion proteins of the nicotinic acetylcholine receptor from mammalian muscle identifies the membrane-spanning regions in the alpha and delta subunits." @default.
- W2038697935 cites W1519445799 @default.
- W2038697935 cites W1550785896 @default.
- W2038697935 cites W1564031035 @default.
- W2038697935 cites W1564893509 @default.
- W2038697935 cites W1568475026 @default.
- W2038697935 cites W1570901130 @default.
- W2038697935 cites W1571025663 @default.
- W2038697935 cites W1572333556 @default.
- W2038697935 cites W1584935191 @default.
- W2038697935 cites W1586011710 @default.
- W2038697935 cites W1596601112 @default.
- W2038697935 cites W1788436564 @default.
- W2038697935 cites W1835219656 @default.
- W2038697935 cites W1939201264 @default.
- W2038697935 cites W1978964533 @default.
- W2038697935 cites W1979185314 @default.
- W2038697935 cites W1988182305 @default.
- W2038697935 cites W1989346371 @default.
- W2038697935 cites W1990315003 @default.
- W2038697935 cites W1991871610 @default.
- W2038697935 cites W1993816915 @default.
- W2038697935 cites W1996916918 @default.
- W2038697935 cites W1999142171 @default.
- W2038697935 cites W2000148336 @default.
- W2038697935 cites W2005560359 @default.
- W2038697935 cites W2008500730 @default.
- W2038697935 cites W2008740919 @default.
- W2038697935 cites W2010311047 @default.
- W2038697935 cites W2012249011 @default.
- W2038697935 cites W2013025891 @default.
- W2038697935 cites W2018289835 @default.
- W2038697935 cites W2019433452 @default.
- W2038697935 cites W2028013850 @default.
- W2038697935 cites W2029766804 @default.
- W2038697935 cites W2031287824 @default.
- W2038697935 cites W2031544074 @default.
- W2038697935 cites W2036931150 @default.
- W2038697935 cites W2039880680 @default.
- W2038697935 cites W2043302566 @default.
- W2038697935 cites W2048602049 @default.
- W2038697935 cites W2050115148 @default.
- W2038697935 cites W2056786659 @default.
- W2038697935 cites W2068120916 @default.
- W2038697935 cites W2068447481 @default.
- W2038697935 cites W2078119356 @default.
- W2038697935 cites W2078935108 @default.
- W2038697935 cites W2081650645 @default.
- W2038697935 cites W2084939728 @default.
- W2038697935 cites W2092574639 @default.
- W2038697935 cites W2095448507 @default.
- W2038697935 cites W2100837269 @default.
- W2038697935 cites W2104224952 @default.
- W2038697935 cites W2108009726 @default.
- W2038697935 cites W2126980491 @default.
- W2038697935 cites W2149745617 @default.
- W2038697935 cites W2165391107 @default.
- W2038697935 cites W2412209475 @default.
- W2038697935 doi "https://doi.org/10.1083/jcb.116.2.385" @default.
- W2038697935 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2289298" @default.
- W2038697935 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1730761" @default.
- W2038697935 hasPublicationYear "1992" @default.
- W2038697935 type Work @default.
- W2038697935 sameAs 2038697935 @default.
- W2038697935 citedByCount "88" @default.
- W2038697935 countsByYear W20386979352012 @default.
- W2038697935 countsByYear W20386979352021 @default.
- W2038697935 crossrefType "journal-article" @default.
- W2038697935 hasAuthorship W2038697935A5028036842 @default.
- W2038697935 hasAuthorship W2038697935A5069247857 @default.
- W2038697935 hasBestOaLocation W20386979351 @default.
- W2038697935 hasConcept C104292427 @default.
- W2038697935 hasConcept C104317684 @default.
- W2038697935 hasConcept C118892022 @default.
- W2038697935 hasConcept C123894998 @default.
- W2038697935 hasConcept C144647389 @default.
- W2038697935 hasConcept C153911025 @default.
- W2038697935 hasConcept C170493617 @default.
- W2038697935 hasConcept C181199279 @default.
- W2038697935 hasConcept C24530287 @default.
- W2038697935 hasConcept C2779064019 @default.
- W2038697935 hasConcept C2779570518 @default.
- W2038697935 hasConcept C2781031661 @default.
- W2038697935 hasConcept C2781307694 @default.
- W2038697935 hasConcept C40767141 @default.
- W2038697935 hasConcept C41625074 @default.
- W2038697935 hasConcept C55493867 @default.
- W2038697935 hasConcept C71315377 @default.
- W2038697935 hasConcept C80161118 @default.
- W2038697935 hasConcept C83730767 @default.
- W2038697935 hasConcept C86803240 @default.
- W2038697935 hasConcept C95444343 @default.