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• W2038715644 endingPage "1457" @default.
• W2038715644 startingPage "1449" @default.
• W2038715644 abstract "Here we describe an algorithm for identifying peptides/ proteins of known sequence and unknown peptides from partial spectra generated by an in-source decay (ISD) technique coupled with matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry. The identification of protein fragments is processed with a software program called CMATCH, which generates candidate subsequences for both known peptides/proteins and unknown peptides for the major product ions in the spectral range m/z 400-5000 and then matches these to known protein sequences contained in a reference database for the known peptides/proteins. CMATCH, which is compiled for MSDOS or WINDOWS95/NT, has two main advantages: first, the candidate subsequences are generated automatically without the need for supplementary information concerning the distribution of either N-terminal or C-terminal ions in the spectra for both known peptides/proteins and unknown peptides; second, the highest coordinated homologous sequences are picked up automatically from the reference database as the best matches with known peptides/proteins. Examples from the ISD spectra of several test proteins demonstrate the efficacy of this protein identification software." @default.
• W2038715644 created "2016-06-24" @default.
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• W2038715644 date "2002-02-12" @default.
• W2038715644 modified "2023-10-09" @default.
• W2038715644 title "A Programmable Fragmentation Analysis of Proteins by In-Source Decay in MALDI-TOF Mass Spectrometry" @default.
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• W2038715644 doi "https://doi.org/10.1021/ac010900d" @default.
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