FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2038872087> ?p ?o ?g. }

• W2038872087 endingPage "18" @default.
• W2038872087 startingPage "13" @default.
• W2038872087 abstract "The spatial control of cells on a surface and the patterning of multiple cell types is an important tool for fundamental biological research and tissue engineering applications. A novel technique is described for the controlled seeding of multiple cell types at specific locations on a surface without requiring the use of specialized equipment or materials. Small-volume, quasi-hemispherical drops of cell solution are deposited onto a cell culture surface immersed under barrier oil, which serves to contain the drop and prevents evaporation of the cell culture medium during the time necessary for cells to attach to the cell culture surface. Subsequent flooding with an aqueous cell-compatible buffer displaces the barrier oil, allowing the cells to grow freely across the surface. This technique offers a simple and easily implemented solution for defining the initial position of cultured cells. The coculture of multiple cell types may be carried out by incorporating different cell types in each drop. A suitable drop volume was found to be 1 microl dispensed with a standard 0.5-10 microl pipette. The drop formed resulted in a footprint diameter of approximately 2 mm. Mineral oil and silicone oil do not compromise the viability of cultured cells when used in this technique. Moreover, a surface with heparin-immobilized FGF2 is shown to retain its bioactivity following drying of the substrate and contact with mineral oil." @default.
• W2038872087 created "2016-06-24" @default.
• W2038872087 creator A5045439116 @default.
• W2038872087 creator A5051670190 @default.
• W2038872087 creator A5061312982 @default.
• W2038872087 date "2009-04-30" @default.
• W2038872087 modified "2023-09-23" @default.
• W2038872087 title "A novel technique for positioning multiple cell types by liquid handling" @default.
• W2038872087 cites W153985519 @default.
• W2038872087 cites W1698518057 @default.
• W2038872087 cites W1973076220 @default.
• W2038872087 cites W1983967894 @default.
• W2038872087 cites W1984267955 @default.
• W2038872087 cites W1986161925 @default.
• W2038872087 cites W1990612468 @default.
• W2038872087 cites W2010229362 @default.
• W2038872087 cites W2028421235 @default.
• W2038872087 cites W2030410920 @default.
• W2038872087 cites W2035444054 @default.
• W2038872087 cites W2043938126 @default.
• W2038872087 cites W2051802591 @default.
• W2038872087 cites W2059466663 @default.
• W2038872087 cites W2064586787 @default.
• W2038872087 cites W2066772900 @default.
• W2038872087 cites W2076407216 @default.
• W2038872087 cites W2102674766 @default.
• W2038872087 cites W2122892477 @default.
• W2038872087 cites W2137448801 @default.
• W2038872087 cites W2168721209 @default.
• W2038872087 cites W4233594877 @default.
• W2038872087 doi "https://doi.org/10.1116/1.3122025" @default.
• W2038872087 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20408718" @default.
• W2038872087 hasPublicationYear "2009" @default.
• W2038872087 type Work @default.
• W2038872087 sameAs 2038872087 @default.
• W2038872087 citedByCount "6" @default.
• W2038872087 countsByYear W20388720872015 @default.
• W2038872087 countsByYear W20388720872017 @default.
• W2038872087 countsByYear W20388720872019 @default.
• W2038872087 countsByYear W20388720872020 @default.
• W2038872087 crossrefType "journal-article" @default.
• W2038872087 hasAuthorship W2038872087A5045439116 @default.
• W2038872087 hasAuthorship W2038872087A5051670190 @default.
• W2038872087 hasAuthorship W2038872087A5061312982 @default.
• W2038872087 hasBestOaLocation W20388720871 @default.
• W2038872087 hasConcept C127413603 @default.
• W2038872087 hasConcept C136229726 @default.
• W2038872087 hasConcept C145018004 @default.
• W2038872087 hasConcept C147789679 @default.
• W2038872087 hasConcept C1491633281 @default.
• W2038872087 hasConcept C151552171 @default.
• W2038872087 hasConcept C159985019 @default.
• W2038872087 hasConcept C171250308 @default.
• W2038872087 hasConcept C185592680 @default.
• W2038872087 hasConcept C192562407 @default.
• W2038872087 hasConcept C2775952280 @default.
• W2038872087 hasConcept C2781345722 @default.
• W2038872087 hasConcept C41008148 @default.
• W2038872087 hasConcept C42360764 @default.
• W2038872087 hasConcept C43617362 @default.
• W2038872087 hasConcept C55493867 @default.
• W2038872087 hasConcept C71924100 @default.
• W2038872087 hasConcept C76155785 @default.
• W2038872087 hasConceptScore W2038872087C127413603 @default.
• W2038872087 hasConceptScore W2038872087C136229726 @default.
• W2038872087 hasConceptScore W2038872087C145018004 @default.
• W2038872087 hasConceptScore W2038872087C147789679 @default.
• W2038872087 hasConceptScore W2038872087C1491633281 @default.
• W2038872087 hasConceptScore W2038872087C151552171 @default.
• W2038872087 hasConceptScore W2038872087C159985019 @default.
• W2038872087 hasConceptScore W2038872087C171250308 @default.
• W2038872087 hasConceptScore W2038872087C185592680 @default.
• W2038872087 hasConceptScore W2038872087C192562407 @default.
• W2038872087 hasConceptScore W2038872087C2775952280 @default.
• W2038872087 hasConceptScore W2038872087C2781345722 @default.
• W2038872087 hasConceptScore W2038872087C41008148 @default.
• W2038872087 hasConceptScore W2038872087C42360764 @default.
• W2038872087 hasConceptScore W2038872087C43617362 @default.
• W2038872087 hasConceptScore W2038872087C55493867 @default.
• W2038872087 hasConceptScore W2038872087C71924100 @default.
• W2038872087 hasConceptScore W2038872087C76155785 @default.
• W2038872087 hasIssue "2" @default.
• W2038872087 hasLocation W20388720871 @default.
• W2038872087 hasLocation W20388720872 @default.
• W2038872087 hasOpenAccess W2038872087 @default.
• W2038872087 hasPrimaryLocation W20388720871 @default.
• W2038872087 hasRelatedWork W2001167147 @default.
• W2038872087 hasRelatedWork W2038872087 @default.
• W2038872087 hasRelatedWork W2050237276 @default.
• W2038872087 hasRelatedWork W2054273181 @default.
• W2038872087 hasRelatedWork W2070483617 @default.
• W2038872087 hasRelatedWork W2557430545 @default.
• W2038872087 hasRelatedWork W2743194952 @default.
• W2038872087 hasRelatedWork W2748952813 @default.
• W2038872087 hasRelatedWork W2899084033 @default.
• W2038872087 hasRelatedWork W2740168187 @default.
• W2038872087 hasVolume "4" @default.
• W2038872087 isParatext "false" @default.

• next