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- W2038893921 abstract "Abstract A convenient method for distinguishing underivatized isomeric monosaccharides has been established using electrospray ionization ion trap mass spectrometry (ESI‐ITMS). Mass spectra of hexoses (glucose, galactose, and mannose), N ‐acetylhexosamines ( N ‐acetylglucosamine, N ‐acetylgalactosamine, and N ‐acetylmannosamine) and hexosamines (glucosamine, galactosamine, and mannosamine) dissolved in solvent containing 1 mM ammonium acetate were obtained in the positive ion mode. Glucose was distinguished from galactose and mannose in the MS 2 spectrum of the [M+NH 4 ] + ion at m/z 198. The MS 3 spectra generated from [M+NH 4 –H 2 O–NH 3 ] + at m/z 163 showed that galactose and mannose could be distinguished by the ratio of peak intensities at m/z 145 and 127, while the three N ‐acetylhexosamine and hexosamine stereochemical isomers could be identified by the relative abundance ratios of product ions observed in MS 3 spectra. The investigation of MS and MS 2 spectra from complexes of these monosaccharides with Na + and Pb 2+ failed to distinguish these monosaccharide isomers. Therefore, multiple stage mass analysis by ESI‐ITMS using either [M+NH 4 ] + or [M+H] + was useful to distinguish between the isomers of monosaccharides. Copyright © 2006 John Wiley & Sons, Ltd." @default.
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- W2038893921 date "2006-12-15" @default.
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- W2038893921 title "The distinction of underivatized monosaccharides using electrospray ionization ion trap mass spectrometry" @default.
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- W2038893921 doi "https://doi.org/10.1002/rcm.2825" @default.
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