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- W2038962007 abstract "The DNA polymerase of intact trout liver nuclei behaved similarly to that present in mammalian tissues in its mandatory requirement for four deoxynucleoside triphosphates, Mg 2+ , and DNA primer. It was inhibited by DNase and pyrophosphate in comparatively low concentrations and by RNase and Actinomycin D in comparatively high concentrations. Soluble DNA polymerase was extracted from buffered suspensions of the nuclei by centrifugation at 100,000 g. It possessed properties very similar to those exhibited by the enzyme in intact nuclei. The polymerase activity of intact nuclei was increased by freezing and thawing, and the soluble enzyme was unstable at 30 C and lost most of its activity in 1 day at 0 C. The specific activity of the enzyme was 10–29 picomoles of deoxynucleoside triphosphate incorporated into DNA by 1 mg of protein in 1 hr at 25 C." @default.
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- W2038962007 modified "2023-10-14" @default.
- W2038962007 title "Deoxyribonucleic Acid Polymerase of Trout Liver Nuclei" @default.
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- W2038962007 doi "https://doi.org/10.1139/f70-012" @default.
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