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- W2038978792 abstract "Rohament P, a macerating enzyme preparation from Aspergillus alleaceus containing an endo-polygalacturonase as the major activity but also substantial amounts of cellulase(s), was purified by affinity chromatography on Avicel cellulose. Treating Rohament P with Avicel at different protein:cellulose ratios was more efficient in columns than in batch. An Avicel column, with an enzyme:substrate ratio of 1:80, retained 94% of cellulase from Rohament P in 60 min at 40–45°C, pH 4.4. Treated enzyme containing 6% residual cellulase, when incubated with fresh carrot rasps, released the maximum amount of cells with intact cell walls. Untreated enzyme did not macerate the carrot tissue but liquified under same conditions. Degradation of a washed carrot preparation by treated enzyme was 44% compared with 55% for the untreated enzyme. Gas chromatographic analysis of sugars revealed that treated Rohament P liberated less glucose and others sugars than did untreated enzyme. Enzyme visualization studies of treated and untreated Rohament P reflected a quantitative difference in protein bands in a pH gradient of 4–6 in miniature isoelectric focusing. Application of treated and untreated Rohament P to disks of carrot tissue led to ultrastructural changes. Untreated Rohament P dissolved the middle lamella and disintegrated the fibrillar material predominantly throughout the cell wall, resulting mainly in cell breakage. Treated Rohament P preferentially dissolved the middle lamellar region of the cell wall without touching the fibrillar part. This indicates that pectin is confined to the middle lamellar region." @default.
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- W2038978792 date "1986-09-01" @default.
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- W2038978792 title "The effect of removing cellulase(s) from a commercial pectinase on maceration and liquefaction of carrots" @default.
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- W2038978792 doi "https://doi.org/10.1016/0168-1656(86)90037-4" @default.
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