FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2038992687> ?p ?o ?g. }

• W2038992687 endingPage "3381" @default.
• W2038992687 startingPage "3369" @default.
• W2038992687 abstract "ABSTRACT Extracellular polysaccharides of many bacteria are synthesized by the Wzy polymerase-dependent mechanism, where long-chain polymers are assembled from undecaprenyl-phosphate-linked repeat units on the outer face of the cytoplasmic membrane. In gram-positive bacteria, Wzy-dependent capsules remain largely cell associated via membrane and peptidoglycan linkages. Like many Wzy-dependent capsules, the Streptococcus pneumoniae serotype 2 capsule is branched. In this study, we found that deletions of cps2K , cps2J , or cps2H , which encode a UDP-glucose dehydrogenase necessary for side chain synthesis, the putative Wzx transporter (flippase), and the putative Wzy polymerase, respectively, were obtained only in the presence of suppressor mutations. Most of the suppressor mutations were in cps2E , which encodes the initiating glycosyltransferase for capsule synthesis. The cps2K mutants containing the suppressor mutations produced low levels of high-molecular-weight polymer that was detected only in membrane fractions. cps2K -repaired mutants exhibited only modest increases in capsule production due to the effect of the secondary mutation, but capsule was detectable in both membrane and cell wall fractions. Lethality of the cps2K , cps2J , and cps2H mutations was likely due to sequestration of undecaprenyl-phosphate in the capsule pathway and either preclusion of its turnover for utilization in essential pathways or destabilization of the membrane due to an accumulation of lipid-linked intermediates. The results demonstrate that proper polymer assembly requires not only a functional transporter and polymerase but also complete repeat units. A central role for the initiating glycosyltransferase in controlling capsule synthesis is also suggested." @default.
• W2038992687 created "2016-06-24" @default.
• W2038992687 creator A5019729277 @default.
• W2038992687 creator A5073160339 @default.
• W2038992687 date "2007-05-01" @default.
• W2038992687 modified "2023-10-15" @default.
• W2038992687 title "Mutations Blocking Side Chain Assembly, Polymerization, or Transport of a Wzy-Dependent <i>Streptococcus pneumoniae</i> Capsule Are Lethal in the Absence of Suppressor Mutations and Can Affect Polymer Transfer to the Cell Wall" @default.
• W2038992687 cites W1132588713 @default.
• W2038992687 cites W1483346907 @default.
• W2038992687 cites W1485264912 @default.
• W2038992687 cites W1491692649 @default.
• W2038992687 cites W1492678853 @default.
• W2038992687 cites W1516644194 @default.
• W2038992687 cites W1523447849 @default.
• W2038992687 cites W1548044542 @default.
• W2038992687 cites W1552456029 @default.
• W2038992687 cites W1581672790 @default.
• W2038992687 cites W1598109190 @default.
• W2038992687 cites W1599080309 @default.
• W2038992687 cites W1636195124 @default.
• W2038992687 cites W1759761157 @default.
• W2038992687 cites W1854736465 @default.
• W2038992687 cites W1927748861 @default.
• W2038992687 cites W1943872953 @default.
• W2038992687 cites W1967386006 @default.
• W2038992687 cites W1976030780 @default.
• W2038992687 cites W1977950017 @default.
• W2038992687 cites W1994666573 @default.
• W2038992687 cites W1997124026 @default.
• W2038992687 cites W1999652145 @default.
• W2038992687 cites W2001868240 @default.
• W2038992687 cites W2004187431 @default.
• W2038992687 cites W2006759313 @default.
• W2038992687 cites W2006902603 @default.
• W2038992687 cites W2009921642 @default.
• W2038992687 cites W2011862459 @default.
• W2038992687 cites W2018454041 @default.
• W2038992687 cites W2023964275 @default.
• W2038992687 cites W2041310231 @default.
• W2038992687 cites W2047501687 @default.
• W2038992687 cites W2049085072 @default.
• W2038992687 cites W2051044535 @default.
• W2038992687 cites W2051235227 @default.
• W2038992687 cites W2060797724 @default.
• W2038992687 cites W2063252040 @default.
• W2038992687 cites W2064808095 @default.
• W2038992687 cites W2088385924 @default.
• W2038992687 cites W2095730944 @default.
• W2038992687 cites W2103516617 @default.
• W2038992687 cites W2108608900 @default.
• W2038992687 cites W2108901020 @default.
• W2038992687 cites W2111631374 @default.
• W2038992687 cites W2112260735 @default.
• W2038992687 cites W2113099409 @default.
• W2038992687 cites W2115908628 @default.
• W2038992687 cites W2117319600 @default.
• W2038992687 cites W2117606701 @default.
• W2038992687 cites W2118701157 @default.
• W2038992687 cites W2122609980 @default.
• W2038992687 cites W2127243759 @default.
• W2038992687 cites W2129202145 @default.
• W2038992687 cites W2129312219 @default.
• W2038992687 cites W2134730712 @default.
• W2038992687 cites W2135410970 @default.
• W2038992687 cites W2135965844 @default.
• W2038992687 cites W2137904284 @default.
• W2038992687 cites W2138103240 @default.
• W2038992687 cites W2142384764 @default.
• W2038992687 cites W2144240057 @default.
• W2038992687 cites W2147443360 @default.
• W2038992687 cites W2151622660 @default.
• W2038992687 cites W2156659808 @default.
• W2038992687 cites W2159920973 @default.
• W2038992687 cites W2167435540 @default.
• W2038992687 cites W2169161684 @default.
• W2038992687 cites W2172147965 @default.
• W2038992687 cites W2204771585 @default.
• W2038992687 cites W4234317413 @default.
• W2038992687 cites W4241057414 @default.
• W2038992687 doi "https://doi.org/10.1128/jb.01938-06" @default.
• W2038992687 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1855910" @default.
• W2038992687 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17322316" @default.
• W2038992687 hasPublicationYear "2007" @default.
• W2038992687 type Work @default.
• W2038992687 sameAs 2038992687 @default.
• W2038992687 citedByCount "98" @default.
• W2038992687 countsByYear W20389926872012 @default.
• W2038992687 countsByYear W20389926872013 @default.
• W2038992687 countsByYear W20389926872014 @default.
• W2038992687 countsByYear W20389926872015 @default.
• W2038992687 countsByYear W20389926872016 @default.
• W2038992687 countsByYear W20389926872017 @default.
• W2038992687 countsByYear W20389926872018 @default.
• W2038992687 countsByYear W20389926872019 @default.
• W2038992687 countsByYear W20389926872020 @default.
• W2038992687 countsByYear W20389926872021 @default.
• W2038992687 countsByYear W20389926872022 @default.
• W2038992687 countsByYear W20389926872023 @default.

• next