Matches in SemOpenAlex for { <https://semopenalex.org/work/W2039084329> ?p ?o ?g. }
Showing items 1 to 80 of
80
with 100 items per page.
- W2039084329 endingPage "478" @default.
- W2039084329 startingPage "473" @default.
- W2039084329 abstract "Binding of the Lac repressor to its operator DNA controls the expression of the genes of thelacoperon ofEscherichia coli. Lac repressor's affinity for thelacoperator is diminished by an inducer that affects the structure of the repressor tetramer. Here we report the cloning and sequencing of the mutant Lac repressoritgene, whose product, the LacRtrepressor, shows a higher affinity for the inducer isopropyl-β-D-thiogalactopyranoside (IPTG) and a lower affinity for thelacoperator than the wild-type repressor. We show that the altered phenotype is due to a single amino acid residue replacement; the alanine residue at position 110 in the wild-type is replaced by threonine init. Other amino acid residues in position 110 have been shown to result in aniSphenotype. For theiS-substitution of alanine 110 with lysine we demonstrate an increase in the affinity for operator DNA and a decrease in the affinity for IPTG. Thus, A110→K shows the opposite effect to A110→T on the repressor protein. We explain the phenotype of the LacR mutants by displacements of the conformational equilibrium for the dimeric repressor unit between RR (high operator affinity, low inducer affinity) and R*R* (low operator affinity, high inducer affinity) towards R*R* in theitand towards RR in theiSmutant in position 110 with respect to the wild-type. The putative structures of the wild-type and mutant Lac repressors confirm this conclusion." @default.
- W2039084329 created "2016-06-24" @default.
- W2039084329 creator A5000339271 @default.
- W2039084329 creator A5069960844 @default.
- W2039084329 date "1996-04-01" @default.
- W2039084329 modified "2023-10-18" @default.
- W2039084329 title "The Side-chain of the Amino Acid Residue in Position 110 of the Lac Repressor Influences its Allosteric Equilibrium" @default.
- W2039084329 doi "https://doi.org/10.1006/jmbi.1996.0176" @default.
- W2039084329 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8648615" @default.
- W2039084329 hasPublicationYear "1996" @default.
- W2039084329 type Work @default.
- W2039084329 sameAs 2039084329 @default.
- W2039084329 citedByCount "24" @default.
- W2039084329 countsByYear W20390843292012 @default.
- W2039084329 countsByYear W20390843292014 @default.
- W2039084329 countsByYear W20390843292016 @default.
- W2039084329 countsByYear W20390843292018 @default.
- W2039084329 countsByYear W20390843292020 @default.
- W2039084329 countsByYear W20390843292023 @default.
- W2039084329 crossrefType "journal-article" @default.
- W2039084329 hasAuthorship W2039084329A5000339271 @default.
- W2039084329 hasAuthorship W2039084329A5069960844 @default.
- W2039084329 hasConcept C104317684 @default.
- W2039084329 hasConcept C113629113 @default.
- W2039084329 hasConcept C143065580 @default.
- W2039084329 hasConcept C150194340 @default.
- W2039084329 hasConcept C158448853 @default.
- W2039084329 hasConcept C166342909 @default.
- W2039084329 hasConcept C17020691 @default.
- W2039084329 hasConcept C170344550 @default.
- W2039084329 hasConcept C181199279 @default.
- W2039084329 hasConcept C185592680 @default.
- W2039084329 hasConcept C207583985 @default.
- W2039084329 hasConcept C2225953 @default.
- W2039084329 hasConcept C2779856020 @default.
- W2039084329 hasConcept C2781338088 @default.
- W2039084329 hasConcept C515207424 @default.
- W2039084329 hasConcept C547475151 @default.
- W2039084329 hasConcept C55493867 @default.
- W2039084329 hasConcept C71240020 @default.
- W2039084329 hasConceptScore W2039084329C104317684 @default.
- W2039084329 hasConceptScore W2039084329C113629113 @default.
- W2039084329 hasConceptScore W2039084329C143065580 @default.
- W2039084329 hasConceptScore W2039084329C150194340 @default.
- W2039084329 hasConceptScore W2039084329C158448853 @default.
- W2039084329 hasConceptScore W2039084329C166342909 @default.
- W2039084329 hasConceptScore W2039084329C17020691 @default.
- W2039084329 hasConceptScore W2039084329C170344550 @default.
- W2039084329 hasConceptScore W2039084329C181199279 @default.
- W2039084329 hasConceptScore W2039084329C185592680 @default.
- W2039084329 hasConceptScore W2039084329C207583985 @default.
- W2039084329 hasConceptScore W2039084329C2225953 @default.
- W2039084329 hasConceptScore W2039084329C2779856020 @default.
- W2039084329 hasConceptScore W2039084329C2781338088 @default.
- W2039084329 hasConceptScore W2039084329C515207424 @default.
- W2039084329 hasConceptScore W2039084329C547475151 @default.
- W2039084329 hasConceptScore W2039084329C55493867 @default.
- W2039084329 hasConceptScore W2039084329C71240020 @default.
- W2039084329 hasIssue "3" @default.
- W2039084329 hasLocation W20390843291 @default.
- W2039084329 hasLocation W20390843292 @default.
- W2039084329 hasOpenAccess W2039084329 @default.
- W2039084329 hasPrimaryLocation W20390843291 @default.
- W2039084329 hasRelatedWork W1857623540 @default.
- W2039084329 hasRelatedWork W1968822904 @default.
- W2039084329 hasRelatedWork W2035116306 @default.
- W2039084329 hasRelatedWork W2051280575 @default.
- W2039084329 hasRelatedWork W2055437194 @default.
- W2039084329 hasRelatedWork W2055802725 @default.
- W2039084329 hasRelatedWork W2056573287 @default.
- W2039084329 hasRelatedWork W2057481588 @default.
- W2039084329 hasRelatedWork W2160610822 @default.
- W2039084329 hasRelatedWork W80365941 @default.
- W2039084329 hasVolume "257" @default.
- W2039084329 isParatext "false" @default.
- W2039084329 isRetracted "false" @default.
- W2039084329 magId "2039084329" @default.
- W2039084329 workType "article" @default.