FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2039091519> ?p ?o ?g. }

• W2039091519 endingPage "440" @default.
• W2039091519 startingPage "433" @default.
• W2039091519 abstract "Serine hydroxymethyltransferase (SHMT) catalyzes the inter conversion of serine and tetrahydrofolate (H4-folate) to form glycine and 5,10-methylene H4-folate and generates one-carbon fragments for the synthesis of nucleotides, methionine, thymidylate, choline, etc. In spite of being an indispensable enzyme of the thymidylate cycle, SHMT in Leishmania donovani remains uncharacterized. The study of L. donovani SHMT (ldSHMT) becomes important as this gene is preferentially expressed in the amastigote stage of parasite, which resides in human macrophages. Here we report cloning, expression and purification of a catalytically active ldSHMT. The homogeneity of recombinant protein was analyzed by denaturing gel electrophoresis and protein was found to be 95% pure having yield of 1 mg/l. The recombinant protein is a tetramer of 216 kDa as evidenced by gel filtration chromatography and uses serine and tetrahydrofolate as substrates with Km of 1.6 and 2.4 mM, respectively. Further biochemical studies revealed that pH optimum of ldSHMT is 7.8 and enzyme is thermally stable up to 45 °C. ldSHMT was found sensitive towards denaturants as manifested by loss of enzyme activity at the concentration of 1 M urea or 0.25 M guanidine hydrochloride. This is the first report of purification and characterization of recombinant SHMT from any protozoan source. Studies on recombinant ldSHMT will help in evaluating this enzyme as potential drug target." @default.
• W2039091519 created "2016-06-24" @default.
• W2039091519 creator A5058839581 @default.
• W2039091519 creator A5062274882 @default.
• W2039091519 date "2007-04-01" @default.
• W2039091519 modified "2023-09-26" @default.
• W2039091519 title "Molecular cloning and biochemical characterization of Leishmania donovani serine hydroxymethyltransferase" @default.
• W2039091519 cites W1520840855 @default.
• W2039091519 cites W1563081877 @default.
• W2039091519 cites W1570142440 @default.
• W2039091519 cites W1966793804 @default.
• W2039091519 cites W1969876786 @default.
• W2039091519 cites W1973359847 @default.
• W2039091519 cites W1977627016 @default.
• W2039091519 cites W1981753496 @default.
• W2039091519 cites W1982952016 @default.
• W2039091519 cites W2004334058 @default.
• W2039091519 cites W2008171507 @default.
• W2039091519 cites W2014476907 @default.
• W2039091519 cites W2014662504 @default.
• W2039091519 cites W2029485026 @default.
• W2039091519 cites W2035224458 @default.
• W2039091519 cites W2044275630 @default.
• W2039091519 cites W2049140110 @default.
• W2039091519 cites W2051262391 @default.
• W2039091519 cites W2060535373 @default.
• W2039091519 cites W2067228752 @default.
• W2039091519 cites W2079504586 @default.
• W2039091519 cites W2082293404 @default.
• W2039091519 cites W2084434937 @default.
• W2039091519 cites W2086827526 @default.
• W2039091519 cites W2087299685 @default.
• W2039091519 cites W2095943555 @default.
• W2039091519 cites W2128913438 @default.
• W2039091519 cites W2140166038 @default.
• W2039091519 cites W2141360780 @default.
• W2039091519 cites W2148589496 @default.
• W2039091519 cites W2152397624 @default.
• W2039091519 cites W2158758064 @default.
• W2039091519 cites W2168799765 @default.
• W2039091519 cites W2279417831 @default.
• W2039091519 cites W2394887674 @default.
• W2039091519 cites W4248396965 @default.
• W2039091519 doi "https://doi.org/10.1016/j.pep.2006.10.013" @default.
• W2039091519 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17142057" @default.
• W2039091519 hasPublicationYear "2007" @default.
• W2039091519 type Work @default.
• W2039091519 sameAs 2039091519 @default.
• W2039091519 citedByCount "12" @default.
• W2039091519 countsByYear W20390915192014 @default.
• W2039091519 countsByYear W20390915192015 @default.
• W2039091519 countsByYear W20390915192017 @default.
• W2039091519 countsByYear W20390915192018 @default.
• W2039091519 countsByYear W20390915192020 @default.
• W2039091519 crossrefType "journal-article" @default.
• W2039091519 hasAuthorship W2039091519A5058839581 @default.
• W2039091519 hasAuthorship W2039091519A5062274882 @default.
• W2039091519 hasConcept C104317684 @default.
• W2039091519 hasConcept C153911025 @default.
• W2039091519 hasConcept C179247698 @default.
• W2039091519 hasConcept C181199279 @default.
• W2039091519 hasConcept C185592680 @default.
• W2039091519 hasConcept C2776414213 @default.
• W2039091519 hasConcept C2779463914 @default.
• W2039091519 hasConcept C2779932687 @default.
• W2039091519 hasConcept C2780912031 @default.
• W2039091519 hasConcept C2909420578 @default.
• W2039091519 hasConcept C40767141 @default.
• W2039091519 hasConcept C515207424 @default.
• W2039091519 hasConcept C55493867 @default.
• W2039091519 hasConcept C86803240 @default.
• W2039091519 hasConceptScore W2039091519C104317684 @default.
• W2039091519 hasConceptScore W2039091519C153911025 @default.
• W2039091519 hasConceptScore W2039091519C179247698 @default.
• W2039091519 hasConceptScore W2039091519C181199279 @default.
• W2039091519 hasConceptScore W2039091519C185592680 @default.
• W2039091519 hasConceptScore W2039091519C2776414213 @default.
• W2039091519 hasConceptScore W2039091519C2779463914 @default.
• W2039091519 hasConceptScore W2039091519C2779932687 @default.
• W2039091519 hasConceptScore W2039091519C2780912031 @default.
• W2039091519 hasConceptScore W2039091519C2909420578 @default.
• W2039091519 hasConceptScore W2039091519C40767141 @default.
• W2039091519 hasConceptScore W2039091519C515207424 @default.
• W2039091519 hasConceptScore W2039091519C55493867 @default.
• W2039091519 hasConceptScore W2039091519C86803240 @default.
• W2039091519 hasIssue "2" @default.
• W2039091519 hasLocation W20390915191 @default.
• W2039091519 hasLocation W20390915192 @default.
• W2039091519 hasOpenAccess W2039091519 @default.
• W2039091519 hasPrimaryLocation W20390915191 @default.
• W2039091519 hasRelatedWork W1535356922 @default.
• W2039091519 hasRelatedWork W1586384817 @default.
• W2039091519 hasRelatedWork W1756352294 @default.
• W2039091519 hasRelatedWork W2014345659 @default.
• W2039091519 hasRelatedWork W2016410782 @default.
• W2039091519 hasRelatedWork W2039091519 @default.
• W2039091519 hasRelatedWork W2089742800 @default.
• W2039091519 hasRelatedWork W2106113567 @default.

• next