FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2039096554> ?p ?o ?g. }

• W2039096554 endingPage "861" @default.
• W2039096554 startingPage "851" @default.
• W2039096554 abstract "To understand the molecular basis of sperm-motility and to identify related novel motility biomarkers.Two-dimensional electrophoresis (2DE) followed by Reverse-phase-nano-high-performance liquid chromatography-electrospray ionization tandem mass spectrometry (RP-nano-HPLC-ESI-MS/MS) were applied to establish the human sperm proteome. Then the sperm proteome of moderate-motile human sperm fraction and that of good-motile human sperm fraction from pooled spermatozoa of forty normozoospermic donors (Group 1 subjects) were compared to identify the dysregulated proteins. Among these down-regulated proteins, Protein tyrosine phosphatase non-receptor type 14 (PTPN14) was chosen to reconfirm by Western blotting and semi-quantitative reverse transcription polymerase chain reaction. For clinical application, Western blotting and real-time reverse transcription polymerase chain reaction was performed to compare the expression level of PTPN14 in (Group 2 subjects) nine normozoospermic controls and thirty-three asthenozoospermic patients (including 21 mild asthenozoospermic cases and 12 severe cases). Finally, bioinformatic tools prediction and immunofluorescence assay were performed to elucidate the potential localization of PTPN14.The expression levels of three proteins were observed to be lower in the moderate-motile sperm fraction than in good-motile sperm of group 1 subjects. Among three proteins with persistent down-regulation in the moderate-motile sperm, we reconfirmed that the expression level of PTPN14 was significantly lower in both mRNA and protein levels from the moderate-motile sperm fraction. Further, down-regulation of PTPN14 was found at the translational and transcriptional level in the asthenozoospermic men. Finally, Bioinformatic tools prediction and immunofluorescence assay showed that PTPN14 maybe predominantly localized at the mitochondria in the midpiece of human ejaculated sperm.Proteomics tools were applied to identify three possible sperm motility-related proteins. Among these proteins, PTPN14 was highly likely a novel sperm-motility biomarker and a potential mitochondrial protein." @default.
• W2039096554 created "2016-06-24" @default.
• W2039096554 creator A5015747095 @default.
• W2039096554 creator A5016970850 @default.
• W2039096554 creator A5018776288 @default.
• W2039096554 creator A5023266702 @default.
• W2039096554 creator A5049312650 @default.
• W2039096554 creator A5074686124 @default.
• W2039096554 date "2011-06-24" @default.
• W2039096554 modified "2023-09-23" @default.
• W2039096554 title "Protein tyrosine phosphatase non-receptor type 14 is a novel sperm-motility biomarker" @default.
• W2039096554 cites W1868238307 @default.
• W2039096554 cites W1870184698 @default.
• W2039096554 cites W1964888298 @default.
• W2039096554 cites W1972616020 @default.
• W2039096554 cites W1975350721 @default.
• W2039096554 cites W1978339348 @default.
• W2039096554 cites W1979034912 @default.
• W2039096554 cites W1984646459 @default.
• W2039096554 cites W1985816784 @default.
• W2039096554 cites W1988934693 @default.
• W2039096554 cites W1996515442 @default.
• W2039096554 cites W1999817659 @default.
• W2039096554 cites W2001463676 @default.
• W2039096554 cites W2019759732 @default.
• W2039096554 cites W2028186423 @default.
• W2039096554 cites W2029068679 @default.
• W2039096554 cites W2030100303 @default.
• W2039096554 cites W2032147742 @default.
• W2039096554 cites W2033431236 @default.
• W2039096554 cites W2038323244 @default.
• W2039096554 cites W2040608972 @default.
• W2039096554 cites W2042202484 @default.
• W2039096554 cites W2043264548 @default.
• W2039096554 cites W2043617798 @default.
• W2039096554 cites W2044297412 @default.
• W2039096554 cites W2046052274 @default.
• W2039096554 cites W2047868904 @default.
• W2039096554 cites W2063651021 @default.
• W2039096554 cites W2071740910 @default.
• W2039096554 cites W2080982938 @default.
• W2039096554 cites W2084034472 @default.
• W2039096554 cites W2099776736 @default.
• W2039096554 cites W2100379229 @default.
• W2039096554 cites W2105055300 @default.
• W2039096554 cites W2105704504 @default.
• W2039096554 cites W2118149443 @default.
• W2039096554 cites W2120898043 @default.
• W2039096554 cites W2120959488 @default.
• W2039096554 cites W2126024920 @default.
• W2039096554 cites W2135885977 @default.
• W2039096554 cites W2148952454 @default.
• W2039096554 cites W2169824343 @default.
• W2039096554 cites W2170537708 @default.
• W2039096554 cites W2171779377 @default.
• W2039096554 cites W245007249 @default.
• W2039096554 cites W4293247451 @default.
• W2039096554 doi "https://doi.org/10.1007/s10815-011-9602-0" @default.
• W2039096554 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3169687" @default.
• W2039096554 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21701840" @default.
• W2039096554 hasPublicationYear "2011" @default.
• W2039096554 type Work @default.
• W2039096554 sameAs 2039096554 @default.
• W2039096554 citedByCount "14" @default.
• W2039096554 countsByYear W20390965542012 @default.
• W2039096554 countsByYear W20390965542013 @default.
• W2039096554 countsByYear W20390965542014 @default.
• W2039096554 countsByYear W20390965542015 @default.
• W2039096554 countsByYear W20390965542016 @default.
• W2039096554 countsByYear W20390965542018 @default.
• W2039096554 countsByYear W20390965542019 @default.
• W2039096554 countsByYear W20390965542020 @default.
• W2039096554 countsByYear W20390965542022 @default.
• W2039096554 crossrefType "journal-article" @default.
• W2039096554 hasAuthorship W2039096554A5015747095 @default.
• W2039096554 hasAuthorship W2039096554A5016970850 @default.
• W2039096554 hasAuthorship W2039096554A5018776288 @default.
• W2039096554 hasAuthorship W2039096554A5023266702 @default.
• W2039096554 hasAuthorship W2039096554A5049312650 @default.
• W2039096554 hasAuthorship W2039096554A5074686124 @default.
• W2039096554 hasBestOaLocation W20390965542 @default.
• W2039096554 hasConcept C104317684 @default.
• W2039096554 hasConcept C104397665 @default.
• W2039096554 hasConcept C105580179 @default.
• W2039096554 hasConcept C153911025 @default.
• W2039096554 hasConcept C16685009 @default.
• W2039096554 hasConcept C2777338322 @default.
• W2039096554 hasConcept C2777632594 @default.
• W2039096554 hasConcept C2777688143 @default.
• W2039096554 hasConcept C2779234561 @default.
• W2039096554 hasConcept C2781087480 @default.
• W2039096554 hasConcept C4692481 @default.
• W2039096554 hasConcept C54355233 @default.
• W2039096554 hasConcept C55493867 @default.
• W2039096554 hasConcept C58207958 @default.
• W2039096554 hasConcept C60644358 @default.
• W2039096554 hasConcept C71924100 @default.
• W2039096554 hasConcept C85265743 @default.

• next