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• W2039154098 startingPage "1037" @default.
• W2039154098 abstract "The elongation factor Tu (EF-Tu) encoded by the tufl gene of the extreme thermophilic bacterium Thermus thermophilus HB8 was expressed under control of the tac promoter from the recombinant plasmid pEFTu-10 in Escherichia coli. Thermophilic EF-Tu-GDP, which amounts to as much as 35% of the cellular protein content, was separated from the E coli EF-Tu-GDP by thermal denaturation at 60 degrees C. The overproduced E coli-born T thermophilus EF-Tu was characterized by: i) recognition through T thermophilus anti-EF-Tu antibodies; ii) analysis of the peptides obtained by cyanogen bromide cleavage; iii) thermostability; iv) guanine nucleotide binding activity in the absence and the presence of elongation factor Ts; and v) ternary complex formation with phenylalanyl-tRNAPhe and GTP." @default.
• W2039154098 created "2016-06-24" @default.
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• W2039154098 date "1991-07-01" @default.
• W2039154098 modified "2023-09-26" @default.
• W2039154098 title "Overproduction of the Thermus thermophilus elongation factor Tu in Escherichia coli" @default.
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• W2039154098 doi "https://doi.org/10.1016/0300-9084(91)90145-q" @default.
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