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- W2039188671 abstract "We previously reported stellate (Ito) cells possess voltage-activated Ca 2+ current. The activation of stellate cells has been indicated to contribute to liver fibrosis and the regulation of hepatic hemodynamics. The aim of this study was to investigate the relationship between voltage-activated Ca 2+ current and activation of stellate cells. Voltage-activated Ca 2+ current in stellate cells isolated from rats were studied using whole-cell patch clamp technique. L-type voltage-activated Ca 2+ current was hardly detected in stellate cells cultured for less than 9 days. Ca 2+ current was detected 12.5 and 69% of cells at the 10th and 14th day of culture, respectively. BrdU incorporation indicated cell proliferation was recognized over 50% of cells at the 3rd and 5th day of culture, respectively, then decreased significantly in a time-dependent manner. On the other hand, the expression of α-smooth muscle actin indicated cell activation increased from 7th day of culture and collagen type I mRNA appeared remarkably in cells cultured for more than 10 days. In this study, we concluded L-type voltage-activated Ca 2+ current was recognized in activated stellate (myofibroblast-like) cells." @default.
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- W2039188671 date "1999-04-01" @default.
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- W2039188671 title "Activated stellate (Ito) cells possess voltage-activated calcium current" @default.
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- W2039188671 doi "https://doi.org/10.1016/s0005-2736(99)00018-8" @default.
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