FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2039197854> ?p ?o ?g. }

• W2039197854 endingPage "11670" @default.
• W2039197854 startingPage "11666" @default.
• W2039197854 abstract "The isocitrate dehydrogenase of Escherichia coli, which lacks the Rossmann fold common to other dehydrogenases, displays a 7000-fold preference for NADP over NAD (calculated as the ratio of kcat/Km). Guided by x-ray crystal structures and molecular modeling, site-directed mutagenesis has been used to introduce six substitutions in the adenosine binding pocket that systematically shift coenzyme preference toward NAD. The engineered enzyme displays an 850-fold preference for NAD over NADP, which exceeds the 140-fold preference displayed by a homologous NAD-dependent enzyme. Of the six mutations introduced, only one is identical in all related NAD-dependent enzyme sequences--strict adherence to homology as a criterion for replacing these amino acids impairs function. Two additional mutations at remote sites improve performance further, resulting in a final mutant enzyme with kinetic characteristics and coenzyme preference comparable to naturally occurring homologous NAD-dependent enzymes." @default.
• W2039197854 created "2016-06-24" @default.
• W2039197854 creator A5002981147 @default.
• W2039197854 creator A5062284449 @default.
• W2039197854 creator A5087169660 @default.
• W2039197854 date "1995-12-05" @default.
• W2039197854 modified "2023-10-18" @default.
• W2039197854 title "A highly active decarboxylating dehydrogenase with rationally inverted coenzyme specificity." @default.
• W2039197854 cites W1550635884 @default.
• W2039197854 cites W1569018409 @default.
• W2039197854 cites W1592200831 @default.
• W2039197854 cites W1983784785 @default.
• W2039197854 cites W2000632075 @default.
• W2039197854 cites W2011039500 @default.
• W2039197854 cites W2018948641 @default.
• W2039197854 cites W2021487571 @default.
• W2039197854 cites W2028961289 @default.
• W2039197854 cites W2040267884 @default.
• W2039197854 cites W2040558316 @default.
• W2039197854 cites W2042037311 @default.
• W2039197854 cites W2049704732 @default.
• W2039197854 cites W2051531989 @default.
• W2039197854 cites W2051719256 @default.
• W2039197854 cites W2062880866 @default.
• W2039197854 cites W2078656511 @default.
• W2039197854 cites W2100498403 @default.
• W2039197854 cites W2132828385 @default.
• W2039197854 cites W2138270253 @default.
• W2039197854 cites W2151001177 @default.
• W2039197854 cites W2888511473 @default.
• W2039197854 doi "https://doi.org/10.1073/pnas.92.25.11666" @default.
• W2039197854 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/40463" @default.
• W2039197854 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8524825" @default.
• W2039197854 hasPublicationYear "1995" @default.
• W2039197854 type Work @default.
• W2039197854 sameAs 2039197854 @default.
• W2039197854 citedByCount "103" @default.
• W2039197854 countsByYear W20391978542012 @default.
• W2039197854 countsByYear W20391978542013 @default.
• W2039197854 countsByYear W20391978542014 @default.
• W2039197854 countsByYear W20391978542015 @default.
• W2039197854 countsByYear W20391978542016 @default.
• W2039197854 countsByYear W20391978542017 @default.
• W2039197854 countsByYear W20391978542018 @default.
• W2039197854 countsByYear W20391978542021 @default.
• W2039197854 countsByYear W20391978542022 @default.
• W2039197854 countsByYear W20391978542023 @default.
• W2039197854 crossrefType "journal-article" @default.
• W2039197854 hasAuthorship W2039197854A5002981147 @default.
• W2039197854 hasAuthorship W2039197854A5062284449 @default.
• W2039197854 hasAuthorship W2039197854A5087169660 @default.
• W2039197854 hasBestOaLocation W20391978541 @default.
• W2039197854 hasConcept C104317684 @default.
• W2039197854 hasConcept C127848430 @default.
• W2039197854 hasConcept C143065580 @default.
• W2039197854 hasConcept C16318435 @default.
• W2039197854 hasConcept C181199279 @default.
• W2039197854 hasConcept C185592680 @default.
• W2039197854 hasConcept C197957613 @default.
• W2039197854 hasConcept C2776317432 @default.
• W2039197854 hasConcept C2777150147 @default.
• W2039197854 hasConcept C2779268744 @default.
• W2039197854 hasConcept C41183919 @default.
• W2039197854 hasConcept C55493867 @default.
• W2039197854 hasConcept C56856141 @default.
• W2039197854 hasConcept C71240020 @default.
• W2039197854 hasConcept C75520062 @default.
• W2039197854 hasConcept C86803240 @default.
• W2039197854 hasConceptScore W2039197854C104317684 @default.
• W2039197854 hasConceptScore W2039197854C127848430 @default.
• W2039197854 hasConceptScore W2039197854C143065580 @default.
• W2039197854 hasConceptScore W2039197854C16318435 @default.
• W2039197854 hasConceptScore W2039197854C181199279 @default.
• W2039197854 hasConceptScore W2039197854C185592680 @default.
• W2039197854 hasConceptScore W2039197854C197957613 @default.
• W2039197854 hasConceptScore W2039197854C2776317432 @default.
• W2039197854 hasConceptScore W2039197854C2777150147 @default.
• W2039197854 hasConceptScore W2039197854C2779268744 @default.
• W2039197854 hasConceptScore W2039197854C41183919 @default.
• W2039197854 hasConceptScore W2039197854C55493867 @default.
• W2039197854 hasConceptScore W2039197854C56856141 @default.
• W2039197854 hasConceptScore W2039197854C71240020 @default.
• W2039197854 hasConceptScore W2039197854C75520062 @default.
• W2039197854 hasConceptScore W2039197854C86803240 @default.
• W2039197854 hasIssue "25" @default.
• W2039197854 hasLocation W20391978541 @default.
• W2039197854 hasLocation W20391978542 @default.
• W2039197854 hasLocation W20391978543 @default.
• W2039197854 hasLocation W20391978544 @default.
• W2039197854 hasOpenAccess W2039197854 @default.
• W2039197854 hasPrimaryLocation W20391978541 @default.
• W2039197854 hasRelatedWork W1834004242 @default.
• W2039197854 hasRelatedWork W1966284598 @default.
• W2039197854 hasRelatedWork W1968108220 @default.
• W2039197854 hasRelatedWork W2029813482 @default.
• W2039197854 hasRelatedWork W2049704732 @default.
• W2039197854 hasRelatedWork W2087600193 @default.
• W2039197854 hasRelatedWork W2590698727 @default.

• next