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- W2039205593 abstract "New systematic approaches are necessary to determine and optimize the chemical and mechanical scaffold properties for hyaline cartilage generation using the limited cell numbers obtained from primary human sources. Peptide functionalized hydrogels possessing continuous variations in physico-chemical properties are an efficient three-dimensional platform for studying several properties simultaneously. Herein, we describe a polyethylene glycol dimethacrylate (PEGDM) hydrogel system possessing a gradient of arginine-glycine-aspartic acid peptide (RGD) concentrations from 0mM to 10mM. The system is used to correlate primary human osteoarthritic chondrocyte proliferation, phenotype maintenance and extracellular matrix (ECM) production to the gradient hydrogel properties. Cell number and chondrogenic phenotype (CD14:CD90 ratios) were found to decline in regions with higher RGD concentrations, while regions with lower RGD concentrations maintained cell number and phenotype. Over three weeks of culture, hydrogel regions containing lower RGD concentrations experience an increase in ECM content compared to regions with higher RGD concentrations. Variations in actin amounts and vinculin organization were observed within the RGD concentration gradients that contribute to the differences in chondrogenic phenotype maintenance and ECM expression." @default.
- W2039205593 created "2016-06-24" @default.
- W2039205593 creator A5022445308 @default.
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- W2039205593 creator A5023884204 @default.
- W2039205593 creator A5036982753 @default.
- W2039205593 creator A5083794153 @default.
- W2039205593 date "2013-07-01" @default.
- W2039205593 modified "2023-10-14" @default.
- W2039205593 title "Maximizing phenotype constraint and extracellular matrix production in primary human chondrocytes using arginine–glycine–aspartate concentration gradient hydrogels" @default.
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- W2039205593 doi "https://doi.org/10.1016/j.actbio.2013.04.005" @default.
- W2039205593 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4819405" @default.
- W2039205593 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23567942" @default.
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