FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2039217550> ?p ?o ?g. }

• W2039217550 endingPage "7157" @default.
• W2039217550 startingPage "7147" @default.
• W2039217550 abstract "Intramolecular disulfide bond formation is promoted in oxidizing extracellular and endoplasmic reticulum compartments and often contributes to protein stability and function. DUOX1 and DUOX2 are distinguished from other members of the NOX protein family by the presence of a unique extracellular N-terminal region. These peroxidase-like domains lack the conserved cysteines that confer structural stability to mammalian peroxidases. Sequence-based structure predictions suggest that the thiol groups present are solvent-exposed on a single protein surface and are too distant to support intramolecular disulfide bond formation. To investigate the role of these thiol residues, we introduced four individual cysteine to glycine mutations in the peroxidase-like domains of both human DUOXs and purified the recombinant proteins. The mutations caused little change in the stabilities of the monomeric proteins, supporting the hypothesis that the thiol residues are solvent-exposed and not involved in disulfide bonds that are critical for structural integrity. However, the ability of the isolated hDUOX1 peroxidase-like domain to dimerize was altered, suggesting a role for these cysteines in protein-protein interactions that could facilitate homodimerization of the peroxidase-like domain or, in the full-length protein, heterodimeric interactions with a maturation protein. When full-length hDUOX1 was expressed in HEK293 cells, the mutations resulted in decreased H2O2 production that correlated with a decreased amount of the enzyme localized to the membrane surface rather than with a loss of activity or with a failure to synthesize the mutant proteins. These results support a role for the cysteine residues in intermolecular disulfide bond formation with the DUOX maturation factor DUOXA1." @default.
• W2039217550 created "2016-06-24" @default.
• W2039217550 creator A5003621282 @default.
• W2039217550 creator A5035762419 @default.
• W2039217550 creator A5046485184 @default.
• W2039217550 creator A5051067996 @default.
• W2039217550 creator A5089063045 @default.
• W2039217550 date "2013-03-01" @default.
• W2039217550 modified "2023-10-14" @default.
• W2039217550 title "Conserved Cysteine Residues Provide a Protein-Protein Interaction Surface in Dual Oxidase (DUOX) Proteins" @default.
• W2039217550 cites W1971215115 @default.
• W2039217550 cites W1975760328 @default.
• W2039217550 cites W1977228682 @default.
• W2039217550 cites W1988464444 @default.
• W2039217550 cites W1989631326 @default.
• W2039217550 cites W1998073026 @default.
• W2039217550 cites W1998640908 @default.
• W2039217550 cites W2008160172 @default.
• W2039217550 cites W2014477663 @default.
• W2039217550 cites W2016627433 @default.
• W2039217550 cites W2018004937 @default.
• W2039217550 cites W2024576544 @default.
• W2039217550 cites W2028343348 @default.
• W2039217550 cites W2029945308 @default.
• W2039217550 cites W2035518450 @default.
• W2039217550 cites W2039562073 @default.
• W2039217550 cites W2044337618 @default.
• W2039217550 cites W2059149560 @default.
• W2039217550 cites W2059240598 @default.
• W2039217550 cites W2062014088 @default.
• W2039217550 cites W2063747968 @default.
• W2039217550 cites W2064226229 @default.
• W2039217550 cites W2064258141 @default.
• W2039217550 cites W2064285590 @default.
• W2039217550 cites W2068401663 @default.
• W2039217550 cites W2072972271 @default.
• W2039217550 cites W2082043922 @default.
• W2039217550 cites W2091611263 @default.
• W2039217550 cites W2093563591 @default.
• W2039217550 cites W2096819939 @default.
• W2039217550 cites W2101692904 @default.
• W2039217550 cites W2122156976 @default.
• W2039217550 cites W2136261717 @default.
• W2039217550 cites W2150130743 @default.
• W2039217550 cites W2151309633 @default.
• W2039217550 cites W2152301430 @default.
• W2039217550 cites W2152770371 @default.
• W2039217550 cites W2155083127 @default.
• W2039217550 cites W2161997291 @default.
• W2039217550 cites W4293247451 @default.
• W2039217550 cites W942401611 @default.
• W2039217550 doi "https://doi.org/10.1074/jbc.m112.414797" @default.
• W2039217550 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3591624" @default.
• W2039217550 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23362256" @default.
• W2039217550 hasPublicationYear "2013" @default.
• W2039217550 type Work @default.
• W2039217550 sameAs 2039217550 @default.
• W2039217550 citedByCount "47" @default.
• W2039217550 countsByYear W20392175502014 @default.
• W2039217550 countsByYear W20392175502015 @default.
• W2039217550 countsByYear W20392175502016 @default.
• W2039217550 countsByYear W20392175502017 @default.
• W2039217550 countsByYear W20392175502018 @default.
• W2039217550 countsByYear W20392175502019 @default.
• W2039217550 countsByYear W20392175502020 @default.
• W2039217550 countsByYear W20392175502021 @default.
• W2039217550 countsByYear W20392175502022 @default.
• W2039217550 countsByYear W20392175502023 @default.
• W2039217550 crossrefType "journal-article" @default.
• W2039217550 hasAuthorship W2039217550A5003621282 @default.
• W2039217550 hasAuthorship W2039217550A5035762419 @default.
• W2039217550 hasAuthorship W2039217550A5046485184 @default.
• W2039217550 hasAuthorship W2039217550A5051067996 @default.
• W2039217550 hasAuthorship W2039217550A5089063045 @default.
• W2039217550 hasBestOaLocation W20392175501 @default.
• W2039217550 hasConcept C104317684 @default.
• W2039217550 hasConcept C143065580 @default.
• W2039217550 hasConcept C158617107 @default.
• W2039217550 hasConcept C162008176 @default.
• W2039217550 hasConcept C181199279 @default.
• W2039217550 hasConcept C185592680 @default.
• W2039217550 hasConcept C204328495 @default.
• W2039217550 hasConcept C2779201268 @default.
• W2039217550 hasConcept C47701112 @default.
• W2039217550 hasConcept C55493867 @default.
• W2039217550 hasConceptScore W2039217550C104317684 @default.
• W2039217550 hasConceptScore W2039217550C143065580 @default.
• W2039217550 hasConceptScore W2039217550C158617107 @default.
• W2039217550 hasConceptScore W2039217550C162008176 @default.
• W2039217550 hasConceptScore W2039217550C181199279 @default.
• W2039217550 hasConceptScore W2039217550C185592680 @default.
• W2039217550 hasConceptScore W2039217550C204328495 @default.
• W2039217550 hasConceptScore W2039217550C2779201268 @default.
• W2039217550 hasConceptScore W2039217550C47701112 @default.
• W2039217550 hasConceptScore W2039217550C55493867 @default.
• W2039217550 hasIssue "10" @default.
• W2039217550 hasLocation W20392175501 @default.
• W2039217550 hasLocation W20392175502 @default.

• next