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• W2039238470 abstract "Talin, consisting of a 47-kDa N-terminal head domain (residues 1–433) and a 190-kDa C-terminal rod domain (residues 434–2541), links integrins to the actin cytoskeleton. We previously reported that the binding stoichiometry of integrin αIIbβ3:talin is ∼2:1. More recently, an integrin binding site has been localized to the talin head domain. In the present study, we identified another integrin binding site at the C-terminal region of the talin rod domain. In a solid phase binding assay, RGD affinity-purified αIIbβ3bound in a dose-dependent manner to microtiter wells coated with the isolated 190-kDa proteolytic fragment of the talin rod domain. Additionally, αIIbβ3 also bound to the talin rod domain captured by 8d4, an anti-talin monoclonal antibody. Polyclonal antibodies raised against a recombinant protein fragment corresponding to the entire talin rod domain (anti-talin-R) inhibited αIIbβ3 binding to intact talin by ∼50% but completely blocked αIIbβ3 binding to the talin rod domain. To localize the integrin binding site, we examined αIIbβ3 binding to recombinant polypeptide fragments corresponding to partial sequences of the talin rod domain. Whereas αIIbβ3 bound effectively to talin-(1075–2541) and talin-(1984–2541), it failed to bind to talin-(434–1076) and talin-(434–1975). Furthermore, the binding of αIIbβ3 to talin-(1984–2541) was inhibited by anti-talin-R. These results indicate that an integrin binding site is located within residues 1984–2541 of the talin rod domain. Thus, talin contains two integrin binding sites, one in the homologous FERM (band four-point-one, ezrin,radixin, moesin) domain and another near its C terminus. Because talin exists as an anti-parallel homodimer in focal adhesions, the two integrin binding sites in the adjacent talin molecules would be in close proximity with each other." @default.
• W2039238470 created "2016-06-24" @default.
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• W2039238470 date "2001-11-01" @default.
• W2039238470 modified "2023-10-11" @default.
• W2039238470 title "Localization of an Integrin Binding Site to the C Terminus of Talin" @default.
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• W2039238470 doi "https://doi.org/10.1074/jbc.m108587200" @default.
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