FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2039239030> ?p ?o ?g. }

• W2039239030 abstract "Improvements in the serological diagnosis of neosporosis are needed to differentiate acute versus chronic Neospora caninum infections. In the present study, N. caninum microneme protein 10 (NcMIC10), similar to other microneme proteins, was shown to be released in a calcium-dependent manner. NcMIC10 may be discharged during active invasion of host cells by the parasite, and thus represent an excellent marker for the diagnosis of neosporosis. In order to test this hypothesis, recombinant NcMIC10 (rNcMIC10) was expressed in Escherichia coli, and polyclonal antibodies were generated against non-overlapping fragments of the protein. A capture ELISA was developed using these antibodies, and was found to be highly accurate and reproducible with a detection range of 10-10,000 pg/ml. The anti-rNcMIC10 antibodies used in this study did not cross-react with the Toxoplasma gondii antigens. NcMIC10 was detected by the ELISA in sera of 9 out of 10 goats (90%) experimentally infected with N. caninum tachyzoites. In general, goats infected with a lower dose (10(4)) of the parasite displayed a peak in NcMIC10 levels between weeks 4 and 5 post infection. Goats infected with a higher parasite dose (10(6)) displayed a more rapid increase in NcMIC10 levels. In most animals, NcMIC10 decreased to undetectable levels by week 6 post infection. This is the first circulating Neospora antigen-based assay which may complement the existing antibody-based assays for a rapid and cost-effective definitive diagnosis of neosporosis in livestock." @default.
• W2039239030 created "2016-06-24" @default.
• W2039239030 creator A5013494616 @default.
• W2039239030 creator A5021117450 @default.
• W2039239030 creator A5021827662 @default.
• W2039239030 creator A5026920618 @default.
• W2039239030 creator A5034092020 @default.
• W2039239030 creator A5034733517 @default.
• W2039239030 creator A5043951691 @default.
• W2039239030 creator A5049185431 @default.
• W2039239030 creator A5061867449 @default.
• W2039239030 creator A5070545407 @default.
• W2039239030 creator A5072472543 @default.
• W2039239030 creator A5082358747 @default.
• W2039239030 creator A5087344620 @default.
• W2039239030 creator A5088064571 @default.
• W2039239030 date "2012-06-01" @default.
• W2039239030 modified "2023-09-29" @default.
• W2039239030 title "Characterization of Neospora caninum microneme protein 10 (NcMIC10) and its potential use as a diagnostic marker for neosporosis" @default.
• W2039239030 cites W1547444821 @default.
• W2039239030 cites W1966987571 @default.
• W2039239030 cites W1978590730 @default.
• W2039239030 cites W1981577958 @default.
• W2039239030 cites W1991831576 @default.
• W2039239030 cites W1994958449 @default.
• W2039239030 cites W1995335956 @default.
• W2039239030 cites W2007154033 @default.
• W2039239030 cites W2022045788 @default.
• W2039239030 cites W2026485126 @default.
• W2039239030 cites W2029208282 @default.
• W2039239030 cites W2044384595 @default.
• W2039239030 cites W2052059236 @default.
• W2039239030 cites W2068437504 @default.
• W2039239030 cites W2071039240 @default.
• W2039239030 cites W2074190759 @default.
• W2039239030 cites W2100577789 @default.
• W2039239030 cites W2115630253 @default.
• W2039239030 cites W2118013877 @default.
• W2039239030 cites W2133222647 @default.
• W2039239030 cites W2138011995 @default.
• W2039239030 cites W2138439665 @default.
• W2039239030 cites W2140432151 @default.
• W2039239030 cites W2145728129 @default.
• W2039239030 cites W2148420646 @default.
• W2039239030 cites W2148896054 @default.
• W2039239030 cites W2170157370 @default.
• W2039239030 cites W2172979522 @default.
• W2039239030 cites W2417601365 @default.
• W2039239030 cites W4231478737 @default.
• W2039239030 doi "https://doi.org/10.1016/j.vetpar.2012.01.003" @default.
• W2039239030 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22284302" @default.
• W2039239030 hasPublicationYear "2012" @default.
• W2039239030 type Work @default.
• W2039239030 sameAs 2039239030 @default.
• W2039239030 citedByCount "18" @default.
• W2039239030 countsByYear W20392390302013 @default.
• W2039239030 countsByYear W20392390302014 @default.
• W2039239030 countsByYear W20392390302015 @default.
• W2039239030 countsByYear W20392390302016 @default.
• W2039239030 countsByYear W20392390302017 @default.
• W2039239030 countsByYear W20392390302018 @default.
• W2039239030 countsByYear W20392390302019 @default.
• W2039239030 countsByYear W20392390302020 @default.
• W2039239030 countsByYear W20392390302021 @default.
• W2039239030 countsByYear W20392390302022 @default.
• W2039239030 crossrefType "journal-article" @default.
• W2039239030 hasAuthorship W2039239030A5013494616 @default.
• W2039239030 hasAuthorship W2039239030A5021117450 @default.
• W2039239030 hasAuthorship W2039239030A5021827662 @default.
• W2039239030 hasAuthorship W2039239030A5026920618 @default.
• W2039239030 hasAuthorship W2039239030A5034092020 @default.
• W2039239030 hasAuthorship W2039239030A5034733517 @default.
• W2039239030 hasAuthorship W2039239030A5043951691 @default.
• W2039239030 hasAuthorship W2039239030A5049185431 @default.
• W2039239030 hasAuthorship W2039239030A5061867449 @default.
• W2039239030 hasAuthorship W2039239030A5070545407 @default.
• W2039239030 hasAuthorship W2039239030A5072472543 @default.
• W2039239030 hasAuthorship W2039239030A5082358747 @default.
• W2039239030 hasAuthorship W2039239030A5087344620 @default.
• W2039239030 hasAuthorship W2039239030A5088064571 @default.
• W2039239030 hasConcept C136764020 @default.
• W2039239030 hasConcept C147483822 @default.
• W2039239030 hasConcept C159047783 @default.
• W2039239030 hasConcept C159654299 @default.
• W2039239030 hasConcept C203014093 @default.
• W2039239030 hasConcept C2778048844 @default.
• W2039239030 hasConcept C2778062946 @default.
• W2039239030 hasConcept C2778128430 @default.
• W2039239030 hasConcept C2779357504 @default.
• W2039239030 hasConcept C2779934537 @default.
• W2039239030 hasConcept C2781023229 @default.
• W2039239030 hasConcept C2781154746 @default.
• W2039239030 hasConcept C3019824283 @default.
• W2039239030 hasConcept C41008148 @default.
• W2039239030 hasConcept C45189115 @default.
• W2039239030 hasConcept C71928629 @default.
• W2039239030 hasConcept C86803240 @default.
• W2039239030 hasConcept C89423630 @default.
• W2039239030 hasConcept C956191 @default.
• W2039239030 hasConceptScore W2039239030C136764020 @default.

• next