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- W2039274280 abstract "Toxoplasma gondiiis a protozoan parasite that infects birds and mammals, including humans.T. gondiiT-263 is an attenuated mutant strain that is being developed as a live vaccine to protect cats from shedding oocysts. A cryopreservation procedure forT. gondiiT-263 bradyzoites has been developed to meet the requirement for product stability. A Me2SO-based procedure for the cryopreservation of tachyzoites was used as a basis for process optimization. A modified cell culture plaque assay was used to determine the effects of selected cryobiological parameters on bradyzoite viability. The major parameters evaluated were: (i) cooling rates; (ii) intermediate plunge temperature; and (iii) thawing and dilution rates and temperatures. The optimized cryopreservation protocol comprised incubation in 12.5% Me2SO and 4% BSA for 30 min at room temperature, cooling at 1°C min−1to −40°C, followed by direct transfer into liquid nitrogen. Rapid thawing (approximately 120°C min−1) followed by slow dilution of cryoprotectant over 15 min resulted in the highest survival. The optimized procedure increased survival 10,000-fold over that obtained using an established tachyzoite protocol. This procedure is to be adapted for the large-scale cryopreservation ofT. gondiiT-263 bradyzoites in individual vaccine doses." @default.
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- W2039274280 date "1996-06-01" @default.
- W2039274280 modified "2023-09-23" @default.
- W2039274280 title "Cryopreservation of an Attenuated Vaccine Strain of the Protozoan ParasiteToxoplasma gondii" @default.
- W2039274280 doi "https://doi.org/10.1006/cryo.1996.0033" @default.
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