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- W2039276677 abstract "Mechanical cues play an important role in bone regeneration and affect production and secretion dynamics of growth factors (GFs) involved in osteogenesis. The in vitro models for investigating the mechanoresponsiveness of the involvement of GFs in osteogenesis are limited to two-dimensional monolayer cell culture studies, which do not effectively embody the physiological interactions with the neighboring cells of different types and the interactions with a natural extracellular matrix. Natural bone formation is a complex process that necessitates the contribution of multiple cell types, physical and chemical cues in a three-dimensional (3D) setting. There is a need for in vitro models that represent the physiological diversity and characteristics of bone formation to realistically study the effects of mechanical cues on this process. In vitro cultures of marrow explants inherently ossify and they embody the multicellular and 3D nature of osteogenesis. Therefore, the aim of this study was to assess the mechanoresponsiveness of the scaffold-free, multicellular, and 3D model of osteogenesis based on inherent marrow ossification and to investigate the effects of mechanical loading on the osteoinductive GF production dynamics of this model. These aims were achieved by (1) culturing rat bone marrow explants for 28 days under basal conditions that facilitate inherent ossification, (2) employing mechanical stimulation (compressive loading) between days 12 and 26, and (3) quantifying the final ossified volume (OV) and the production levels of bone morphogenetic protein-2, vascular endothelial growth factor, insulin-like growth factor-1, and transforming growth factor-β1. The results showed that the final OV of the marrow explants increased by about fourfold in mechanically stimulated samples. Further, mechanical stimulation sustained the production level of vascular endothelial growth factor (starting day 21), which otherwise declined temporally under static conditions. The production levels of insulin-like growth factor-1 and transforming growth factor-β1 were enhanced under the effect of loading after day 21. In addition, significant correlations were observed between the final OV and the levels of GFs analyzed. In conclusion, this study demonstrates that the scaffold-free, multicellular, and 3D model of bone formation based on inherent ossification of marrow tissue is mechanoresponsive and mechanical loading improves in vitro osteogenesis in this model with sustaining or enhancing osteoinductive GF production levels, which otherwise would decline with increasing time." @default.
- W2039276677 created "2016-06-24" @default.
- W2039276677 creator A5019537430 @default.
- W2039276677 creator A5064779540 @default.
- W2039276677 creator A5078624568 @default.
- W2039276677 date "2011-02-01" @default.
- W2039276677 modified "2023-10-12" @default.
- W2039276677 title "Ossifying Bone Marrow Explant Culture as a Three-Dimensional Mechanoresponsive<i>In Vitro</i>Model of Osteogenesis" @default.
- W2039276677 cites W122585317 @default.
- W2039276677 cites W1549257184 @default.
- W2039276677 cites W1583699618 @default.
- W2039276677 cites W1607413909 @default.
- W2039276677 cites W1901287843 @default.
- W2039276677 cites W1966260373 @default.
- W2039276677 cites W1969143454 @default.
- W2039276677 cites W1970023570 @default.
- W2039276677 cites W1970727713 @default.
- W2039276677 cites W1971892663 @default.
- W2039276677 cites W1972222251 @default.
- W2039276677 cites W1976845897 @default.
- W2039276677 cites W1977946489 @default.
- W2039276677 cites W1979968943 @default.
- W2039276677 cites W1980876953 @default.
- W2039276677 cites W1983529547 @default.
- W2039276677 cites W1986469118 @default.
- W2039276677 cites W1987159788 @default.
- W2039276677 cites W1989933525 @default.
- W2039276677 cites W1991824786 @default.
- W2039276677 cites W1992786647 @default.
- W2039276677 cites W2000939770 @default.
- W2039276677 cites W2001084083 @default.
- W2039276677 cites W2001821470 @default.
- W2039276677 cites W2005113880 @default.
- W2039276677 cites W2007564570 @default.
- W2039276677 cites W2010635910 @default.
- W2039276677 cites W2011404595 @default.
- W2039276677 cites W2014481070 @default.
- W2039276677 cites W2015272152 @default.
- W2039276677 cites W2015668670 @default.
- W2039276677 cites W2016490294 @default.
- W2039276677 cites W2020781532 @default.
- W2039276677 cites W2027914516 @default.
- W2039276677 cites W2029810059 @default.
- W2039276677 cites W2030752909 @default.
- W2039276677 cites W2030913739 @default.
- W2039276677 cites W2033807339 @default.
- W2039276677 cites W2034080799 @default.
- W2039276677 cites W2036301144 @default.
- W2039276677 cites W2042767306 @default.
- W2039276677 cites W2045505548 @default.
- W2039276677 cites W2045835452 @default.
- W2039276677 cites W2047294094 @default.
- W2039276677 cites W2049401592 @default.
- W2039276677 cites W2050563961 @default.
- W2039276677 cites W2052229494 @default.
- W2039276677 cites W2056477649 @default.
- W2039276677 cites W2056923629 @default.
- W2039276677 cites W2060202533 @default.
- W2039276677 cites W2060968777 @default.
- W2039276677 cites W2061024977 @default.
- W2039276677 cites W2061100934 @default.
- W2039276677 cites W2062504097 @default.
- W2039276677 cites W2063626613 @default.
- W2039276677 cites W2079590118 @default.
- W2039276677 cites W2081451346 @default.
- W2039276677 cites W2081791895 @default.
- W2039276677 cites W2084620794 @default.
- W2039276677 cites W2084909069 @default.
- W2039276677 cites W2089791526 @default.
- W2039276677 cites W2091895305 @default.
- W2039276677 cites W2092658506 @default.
- W2039276677 cites W2092864146 @default.
- W2039276677 cites W2098444281 @default.
- W2039276677 cites W2100436400 @default.
- W2039276677 cites W2103022676 @default.
- W2039276677 cites W2103279664 @default.
- W2039276677 cites W2103684286 @default.
- W2039276677 cites W2108778569 @default.
- W2039276677 cites W2109293470 @default.
- W2039276677 cites W2116515774 @default.
- W2039276677 cites W2119369991 @default.
- W2039276677 cites W2126545660 @default.
- W2039276677 cites W2127432537 @default.
- W2039276677 cites W2144268541 @default.
- W2039276677 cites W2146235933 @default.
- W2039276677 cites W2152723687 @default.
- W2039276677 cites W2154251974 @default.
- W2039276677 cites W2157962408 @default.
- W2039276677 cites W2159999899 @default.
- W2039276677 cites W2167679212 @default.
- W2039276677 cites W2167944119 @default.
- W2039276677 cites W2168789685 @default.
- W2039276677 cites W2293758038 @default.
- W2039276677 cites W4211160782 @default.
- W2039276677 cites W4247505733 @default.
- W2039276677 cites W4249736061 @default.
- W2039276677 cites W4250416532 @default.
- W2039276677 cites W4295836001 @default.