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- W2039296434 abstract "The Rho small G protein family, consisting of the Rho, Rac, and Cdc42 subfamilies, regulates various cell functions, such as cell shape change, cell motility, and cytokinesis, through reorganization of the actin cytoskeleton. We show here that the Rac and Rho subfamilies furthermore regulate cell–cell adhesion. We prepared MDCK cell lines stably expressing each of dominant active mutants of RhoA (sMDCK-RhoDA), Rac1 (sMDCK-RacDA), and Cdc42 (sMDCK-Cdc42DA) and dominant negative mutants of Rac1 (sMDCK-RacDN) and Cdc42 (sMDCK-Cdc42DN) and analyzed cell adhesion in these cell lines. The actin filaments at the cell–cell adhesion sites markedly increased in sMDCK-RacDA cells, whereas they apparently decreased in sMDCK-RacDN cells, compared with those in wild-type MDCK cells. Both E-cadherin and β-catenin, adherens junctional proteins, at the cell–cell adhesion sites also increased in sMDCK-RacDA cells, whereas both of them decreased in sMDCK-RacDN cells. The detergent solubility assay indicated that the amount of detergent-insoluble E-cadherin increased in sMDCK-RacDA cells, whereas it slightly decreased in sMDCK-RacDN cells, compared with that in wild-type MDCK cells. In sMDCK-RhoDA, -Cdc42DA, and -Cdc42DN cells, neither of these proteins at the cell–cell adhesion sites was apparently affected. ZO-1, a tight junctional protein, was not apparently affected in any of the transformant cell lines. Electron microscopic analysis revealed that sMDCK-RacDA cells tightly made contact with each other throughout the lateral membranes, whereas wild-type MDCK and sMDCK-RacDN cells tightly and linearly made contact at the apical area of the lateral membranes. These results suggest that the Rac subfamily regulates the formation of the cadherin-based cell– cell adhesion. Microinjection of C3 into wild-type MDCK cells inhibited the formation of both the cadherin-based cell–cell adhesion and the tight junction, but microinjection of C3 into sMDCK-RacDA cells showed little effect on the localization of the actin filaments and E-cadherin at the cell–cell adhesion sites. These results suggest that the Rho subfamily is necessary for the formation of both the cadherin-based cell– cell adhesion and the tight junction, but not essential for the Rac subfamily-regulated, cadherin-based cell– cell adhesion." @default.
- W2039296434 created "2016-06-24" @default.
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- W2039296434 date "1997-11-17" @default.
- W2039296434 modified "2023-10-18" @default.
- W2039296434 title "Regulation of Cell–Cell Adhesion by Rac and Rho Small G Proteins in MDCK Cells" @default.
- W2039296434 cites W129191972 @default.
- W2039296434 cites W1503137231 @default.
- W2039296434 cites W1513979636 @default.
- W2039296434 cites W1514066364 @default.
- W2039296434 cites W153332747 @default.
- W2039296434 cites W1534652806 @default.
- W2039296434 cites W1556438409 @default.
- W2039296434 cites W1558257735 @default.
- W2039296434 cites W1558802435 @default.
- W2039296434 cites W1580370212 @default.
- W2039296434 cites W1602846778 @default.
- W2039296434 cites W1642347096 @default.
- W2039296434 cites W1744354425 @default.
- W2039296434 cites W1805425496 @default.
- W2039296434 cites W1881577780 @default.
- W2039296434 cites W1910399711 @default.
- W2039296434 cites W1920794749 @default.
- W2039296434 cites W1954540662 @default.
- W2039296434 cites W1956883206 @default.
- W2039296434 cites W1964912103 @default.
- W2039296434 cites W1971589188 @default.
- W2039296434 cites W1972681225 @default.
- W2039296434 cites W1976704214 @default.
- W2039296434 cites W1979713589 @default.
- W2039296434 cites W1982974412 @default.
- W2039296434 cites W1983941497 @default.
- W2039296434 cites W1989143807 @default.
- W2039296434 cites W1992277590 @default.
- W2039296434 cites W1993285794 @default.
- W2039296434 cites W2007660746 @default.
- W2039296434 cites W2012922609 @default.
- W2039296434 cites W2016753320 @default.
- W2039296434 cites W2018307646 @default.
- W2039296434 cites W2023419501 @default.
- W2039296434 cites W2027015202 @default.
- W2039296434 cites W2027810531 @default.
- W2039296434 cites W2029872541 @default.
- W2039296434 cites W2036145275 @default.
- W2039296434 cites W2039117682 @default.
- W2039296434 cites W2040381955 @default.
- W2039296434 cites W2054613918 @default.
- W2039296434 cites W2056918775 @default.
- W2039296434 cites W2063875318 @default.
- W2039296434 cites W2065813594 @default.
- W2039296434 cites W2067703698 @default.
- W2039296434 cites W2076337034 @default.
- W2039296434 cites W2076601321 @default.
- W2039296434 cites W2080030445 @default.
- W2039296434 cites W2084457841 @default.
- W2039296434 cites W2084699108 @default.
- W2039296434 cites W2088303433 @default.
- W2039296434 cites W2089887078 @default.
- W2039296434 cites W2090676188 @default.
- W2039296434 cites W2093226717 @default.
- W2039296434 cites W2097751889 @default.
- W2039296434 cites W2101778925 @default.
- W2039296434 cites W2102877711 @default.
- W2039296434 cites W2103831817 @default.
- W2039296434 cites W2103842570 @default.
- W2039296434 cites W2106911358 @default.
- W2039296434 cites W2110965039 @default.
- W2039296434 cites W2116615268 @default.
- W2039296434 cites W2119593627 @default.
- W2039296434 cites W2120613121 @default.
- W2039296434 cites W2121666423 @default.
- W2039296434 cites W2125144482 @default.
- W2039296434 cites W2130516208 @default.
- W2039296434 cites W2134073082 @default.
- W2039296434 cites W2155651695 @default.
- W2039296434 cites W2157195501 @default.
- W2039296434 cites W2169019804 @default.
- W2039296434 cites W2176511881 @default.
- W2039296434 cites W2183308769 @default.
- W2039296434 cites W2271386161 @default.
- W2039296434 cites W2328730164 @default.
- W2039296434 cites W2394487438 @default.
- W2039296434 cites W2423632767 @default.
- W2039296434 cites W2433794126 @default.
- W2039296434 doi "https://doi.org/10.1083/jcb.139.4.1047" @default.
- W2039296434 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2139955" @default.
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